Epidermal Growth Factor Protein, Human Recombinant

Beta LifeScience offers Recombinant Human EGF proteins for epithelial cell culture, stem cell research, organoid workflows and EGFR signaling studies. Researchers can compare standard E. coli-expressed, animal component-free, low-endotoxin and GMP-format Human EGF products based on purity, biological activity, endotoxin level and manufacturing requirements.

Compare Human EGF proteins or contact Beta LifeScience for current pack sizes, pricing, lead time and technical documentation.

What Is Recombinant Human EGF Protein?

Quick answer: Recombinant Human EGF protein is a laboratory-produced form of human epidermal growth factor used in epithelial cell culture, stem cell research, cell-proliferation assays, organoid workflows and EGFR signaling studies. Epidermal growth factor is a compact mitogenic protein consisting of 53 amino acids in its mature form. It contains three intramolecular disulfide bonds that help maintain its biologically active structure.

Its official gene symbol is EGF, and its human UniProt accession is P01133. Common names include:

• Epidermal growth factor
• EGF
• Human EGF
• Recombinant EGF
• Urogastrone
• Beta-urogastrone
• Pro-epidermal growth factor

Human recombinant EGF can be produced in E. coli under controlled manufacturing conditions. Recombinant production allows researchers to select a growth factor protein with defined purity, biological activity, endotoxin and formulation specifications.

Compare Recombinant Human EGF Proteins

Beta LifeScience offers several Human EGF formats for routine cell culture, defined-media workflows and process-development research.

Review the current product page, datasheet and lot-specific Certificate of Analysis before experimental use. Specifications and availability can vary according to product and production lot.

E. Coli-Expressed Human EGF for Routine Research

The Recombinant Human EGF Protein, BL-1779NP, is an E. coli-expressed growth factor protein for routine cell culture, proliferation and EGFR signaling research.

Its current specifications include:

• Human EGF
• E. coli expression system
• Asn971–Arg1023 expression range
• Mature 53-amino-acid EGF sequence
• Greater than 95% purity by reducing SDS-PAGE
• Molecular mass of approximately 6.2 kDa
• Endotoxin below 0.01 EU/μg
• Verified biological activity
• Lyophilized formulation
• Research-use-only classification

This product can support research involving epithelial cells, epidermal cells, fibroblasts and other EGFR-responsive cell types. It can also provide a controlled stimulant for receptor activation, cell proliferation, and downstream signaling assays. BL-1779NP is lyophilized from a filtered Tris and sodium chloride solution. Researchers should follow the product-specific instructions for reconstitution, aliquoting and storage.

Animal-Free Human EGF for Defined Cell Culture Workflows

The Recombinant Human EGF Protein, Animal-Free, Active, Low Endotoxin, BEP-0004UL is produced using an animal component-free E. coli system.

Its current product features include:

• Animal component-free production
• Greater than 97% purity by SDS-PAGE
• Biological activity measured using Balb/c 3T3 mouse fibroblast cells
• Endotoxin below 0.01 EU/μg
• Lyophilized formulation
• PBS and trehalose formulation

This format can support defined-media workflows where limiting animal-derived materials aligns with the experimental design. Potential applications include sensitive epithelial cell culture, stem cell research and organoid workflows. Researchers should match the EGF concentration to a validated protocol for their specific cells. Basal medium, cell source, passage number, culture density, extracellular matrix and other growth factors can influence the response.

Tag-Free GMP-Format Human EGF for Process Development

The Recombinant Human Epidermal Growth Factor, Active, GMP, BLC-05995P is an E. coli-expressed, tag-free protein fragment manufactured for GMP-oriented research and process-development workflows.

Its current specifications include:

• E. coli expression system
• Tag-free configuration
• Greater than 98% purity by SDS-PAGE and HPLC
• Endotoxin below 0.01 EU/μg
• Fully biologically active compared with a standard
• ED50 below 0.1 ng/mL in a Balb/c 3T3 proliferation assay
• Specific activity greater than 1.0 × 10⁷ IU/mg
• Asn971–Arg1023 expression range
• Molecular weight of approximately 6.2 kDa
• Lyophilized powder

This product supports research requiring high purity, strong biological activity, tag-free EGF and a GMP-format manufacturing option.

The product is supplied according to the research-use conditions stated in its current documentation. Researchers should verify the product grade, available documentation and permitted use before ordering. Compare available Recombinant Human EGF proteins, review current specifications, or request pricing, pack sizes, lead time and lot-specific documentation from Beta LifeScience.

How to Choose a Human Recombinant EGF Protein

The right EGF protein depends on the cell type, culture protocol, experimental objective and required production format.

For Routine Cell Culture

An active E. coli-expressed Human EGF protein can support many routine epithelial, epidermal and fibroblast culture experiments. Confirm that the product’s activity, purity and endotoxin specifications align with the established protocol. BL-1779NP provides greater than 95% purity and endotoxin below 0.01 EU/μg for general research use.

For Defined or Animal-Free Workflows

An animal component-free EGF format can be selected when the research protocol aims to reduce animal-derived manufacturing inputs. BEP-0004UL combines animal component-free E. coli production, greater than 97% purity and low endotoxin for defined research workflows.

For Sensitive Cell Culture

Endotoxin can influence cell behavior independently of EGF. Sensitive, long-term or inflammation-responsive cultures benefit from careful endotoxin selection. Review the product’s reported endotoxin specification alongside the final EGF concentration used in culture.

For EGFR Signaling Assays

Biologically active EGF is important for receptor-phosphorylation, dose-response and pathway-inhibition experiments. Review the reported activity method to determine whether it aligns with the intended assay.

For Process-Development Research

A GMP-manufactured or GMP-oriented research format can support process-development workflows requiring additional manufacturing controls and technical documentation. The product’s stated grade and permitted use should always be confirmed before ordering.

EGF Applications in Cell Culture and Signaling Research

Human EGF binds EGFR, also known as HER1 or ERBB1. Ligand binding promotes receptor activation and initiates downstream pathways associated with proliferation, survival, differentiation and migration.

EGF-responsive signaling can include:

• RAS–RAF–MEK–ERK
• PI3K–AKT
• JAK–STAT
• PLCγ
• Additional cell-specific signaling networks

Researchers use Recombinant Human EGF protein in several connected applications.

Epithelial and Epidermal Cell Culture

EGF can support research involving epithelial cell proliferation, epidermal cell expansion, keratinocyte culture, migration and tissue-remodeling models. A biologically active, low-endotoxin EGF protein provides a defined growth factor for these applications.

Stem and Progenitor Cell Research

EGF is included in selected neural stem cell, neural progenitor and mesenchymal stem cell research protocols. It may be combined with other growth factors and supplements according to the requirements of the cell population.

The appropriate result depends on:

• Cell source
• Passage number
• Basal medium
• EGF concentration
• Additional growth factors
• Culture density
• Extracellular matrix
• Differentiation schedule

Researchers should follow a validated protocol and optimize conditions only when required.

Organoid Workflows

EGF is commonly included in several organoid culture media. It can support EGFR-dependent expansion when combined with an appropriate extracellular matrix and the other signaling factors specified by the protocol. Animal component-free EGF may be considered when a more defined culture workflow is preferred.

Proliferation and Activity Assays

Active Human EGF can stimulate responsive cells in proliferation assays. Such experiments can evaluate growth-factor activity, receptor function, culture conditions or pathway inhibitors. Balb/c 3T3 fibroblast proliferation is one method used to assess EGF biological activity.

EGFR Activation and Drug-Discovery Research

Recombinant EGF provides a controlled ligand for EGFR activation. Researchers can measure receptor phosphorylation and downstream signaling using Western blotting, ELISA, flow cytometry or other analytical methods. It can also support dose-response, time-course and inhibitor-screening experiments involving EGFR-related pathways.

Available Pack Sizes, Pricing and Bulk Quantities

Available pack sizes, pricing and stock status can differ among EGF products and production lots. Researchers should use the product inquiry form or contact page to confirm current commercial information.

When requesting a quotation, provide:

• Catalog number
• Required quantity
• Number of vials
• Delivery timeline
• Intended application
• Preferred product format
• Required documentation

Bulk quantities, multiple vials from one lot, lot reservation, custom concentration, liquid presentation, additional endotoxin removal or alternative formulation may be available following technical evaluation. Providing complete project information allows the Beta LifeScience team to assess whether a catalog product, bulk order or custom-production option can support the study.

COA, Datasheet and Technical Documentation

Researchers should review the current product page, datasheet and lot-specific Certificate of Analysis before using Human recombinant EGF.

Relevant documentation can include:

• Product identity
• Catalog number
• Species
• Expression system
• Sequence range
• Purity
• Molecular weight
• Endotoxin level
• Biological activity
• Formulation
• Reconstitution instructions
• Storage conditions
• Lot number
• Quality-control method

A lot-specific COA supports experimental traceability by confirming the specifications associated with the supplied material. Contact Beta LifeScience to ask about available COAs, datasheets, pack sizes, pricing and lead time.

Custom Human EGF Protein Options

A standard mature Human EGF protein is suitable for many cell culture and signaling applications. Specialized projects may require a different sequence, expression system, tag, endotoxin specification or formulation. Beta LifeScience provides custom protein expression services for project-specific requirements.

Researchers can submit:

• Target protein and accession number
• Required sequence range
• Preferred expression system
• Tag requirements
• Endotoxin target
• Purity target
• Required quantity
• Intended application

Custom formats and additional manufacturing options are subject to technical evaluation. The team can assess whether an existing catalog product or custom-production workflow is more suitable.

Handling Lyophilized Human EGF Protein

Always follow the instructions for the selected catalog number because formulation, reconstitution and storage conditions can differ.

General handling practices include:

1. Briefly centrifuge the vial before opening.
2. Use the recommended sterile reconstitution solution.
3. Reconstitute at the advised concentration.
4. Mix gently and avoid vigorous vortexing.
5. Divide the solution into single-use aliquots.
6. Store aliquots at the specified temperature.
7. Avoid repeated freeze-thaw cycles.
8. Use sterile, low-protein-binding materials where appropriate.

A small quantity of lyophilized EGF can form a thin or transparent layer inside the vial. The product documentation and lot-specific COA confirm the supplied quantity.

Why Choose Beta LifeScience for Human EGF Protein?

Beta LifeScience provides several Human EGF formats for routine cell culture, defined research workflows and process-development studies.

Researchers can compare:

• Standard E. coli-expressed Human EGF
• Biologically active EGF protein
• Animal component-free production
• Low-endotoxin specifications
• Tag-free formats
• GMP-manufactured research products
• Lyophilized protein formulations
• Bulk or custom options following technical evaluation
• COA and datasheet availability
• Technical product-selection support

Request Recombinant Human EGF Pricing

Choose a Human EGF protein according to your cell type, culture medium, endotoxin requirement, activity needs and production format.

Compare available Recombinant Human EGF proteins, review current specifications, or request pricing, pack sizes, lead time and lot-specific documentation from Beta LifeScience.

For project-specific sequences, expression systems or other specialized requirements, explore.

Frequently Asked Questions

Which Recombinant Human EGF protein should I choose?

Choose according to the cell type, protocol, required purity, endotoxin limit and production format. BL-1779NP supports routine research, BEP-0004UL supports animal component-free workflows, and BLC-05995P provides a tag-free GMP-format option for process-development research.

Is Human EGF expressed in E. coli?

Yes. The Beta LifeScience Human EGF products discussed in this guide are expressed in E. coli, including standard, animal component-free and GMP-format options.

Why is low-endotoxin EGF important?

Endotoxin can independently influence cell behavior. Low-endotoxin EGF supports clearer interpretation in sensitive, long-term and inflammation-responsive cell cultures.

Standard vs animal-free EGF: which is better?

The appropriate option depends on the protocol. Standard active EGF supports many routine experiments, while animal component-free EGF suits research workflows designed to limit animal-derived manufacturing inputs.

Can I order bulk Recombinant Human EGF?

Bulk quantities may be available following technical evaluation. Submit the required catalog number, total quantity, number of vials and delivery timeline for a quotation.

Could you please provide a COA or datasheet?

Yes. Contact Beta LifeScience to request available product datasheets, Certificates of Analysis and lot-specific technical documentation.

Is Recombinant Human EGF for research use only?

The products discussed in this guide are supplied according to the research-use conditions stated in their current documentation. Verify the product grade and permitted use before ordering.