Recombinant Human EGF Protein, Animal-Free, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0004UL
Recombinant Human EGF (W262 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 6 kDa and purity greater than 97%.
Recombinant Human EGF (W262 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 6 kDa and purity greater than 97%.

Recombinant Human EGF Protein, Animal-Free, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0004UL
/
Size

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Product Overview

Product Name Recombinant Human EGF Protein, Animal-Free
Target Symbol EGF
Alternative Names Epidermal Growth Factor, Beta-Urogastrone, Urogastrone, URG, HOMG4
Accession Number P01133
Expression System E. coli using an animal component-free system
Molecular Weight 6.4 kDa (predicted)
Sequence Asn971-Arg1023, with an N-terminal Met
Purity >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining.
Activity Determined by a cell proliferation assay using Balb/c-3T3 mouse fibroblast cells. The ED50 for this effect is typically <100 pg/mL.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.01 EU/1ug of the protein as determined by the LAL method.
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function EGF (Epidermal Growth Factor) is a growth factor that plays several roles related to cell growth, proliferation, and differentiation mediated through binding to its receptor, EGFR (Epidermal Growth Factor Receptor). EGF may be expressed in macrophages, platelets, plasma, milk, urine, saliva, seminal fluid, amniotic fluid and cerebrospinal fluid. Several roles are attributed to EGF such as angiogenesis, epithelial cell growth and maintenance, keratinocyte proliferation, gastrointestinal mucosa integrity, along with neurogenesis and neuronal survival. Due to these wide-ranging functions as a growth factor, recombinant human EGF (rhEGF) is commonly used in cell culture for the maintenance and growth of cell lines, stem cells, and 3-D organoids.
Tissue Specificity EGF expression has been reported in skin, gastrointestinal epithelial tissue, salivary glands, kidney, brain, liver, mammary glands, ovaries and testes.
Cellular Localization Membrane-bound and cleaved soluble protein forms.
Involvement In Disease Dysregulation of EGF is associated with several cancers (lung cancer, breast cancer, colorectal cancer, and head and neck cancer), psoriasis, inflammatory bowel disease (IBD), diabetic nephropathy, polycystic kidney disease, peptic ulcers and impaired wound healing.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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