Recombinant Human TGF-beta 1 Protein, Active

Beta LifeScience SKU/CAT #: BEP-0059

Recombinant Human TGF-beta 1 Protein, Active

Beta LifeScience SKU/CAT #: BEP-0059
/
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Submit an inquiry or email sales for a custom bulk quote. Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Product Name Recombinant Human TGF-beta 1 Protein
Target Symbol TGFB1
Alternative Names Transforming Growth Factor beta 1, TGFB, TGFB1, Prepro-Transforming Growth Factor Beta-1, Latency-Associated Peptide, LAP, CED, DPD1
Accession Number P01137
Expression System E. coli.
Molecular Weight 12.9 kDa (monomer, predicted)
Sequence Ala279-Ser390, with an N-terminal Met
Purity Determined by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
Activity Determined by in-house activity assay
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level Available Upon Request
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function Transforming Growth Factor-beta 1 (TGF-beta 1) is a multifunctional cytokine expressed in virtually all tissue types. Regulation of TGF-beta 1 expression occurs by the proteolytic processing of the preproprotein, where a latent complex consisting of a latency-associated peptide (LAP) homodimer, mature peptide homodimer and latent TGF-beta binding protein (LTBP) form and remain in the extracellular matrix. Interactions with the activation pathway release the mature TGF-beta 1 homodimer that is a ligand for a heterodimeric complex composed of TGF-beta RII and either TGF-beta RI or ALK-1. TGF-beta 1 was initially isolated from platelets where it plays a role in wound healing through proliferation of fibroblasts and activation of pathways for extracellular matrix synthesis of collagens and proteoglycans. TGF-beta 1 is involved in the regulation of the immune response through the induction of Th17 or Treg cells based on concentrations within the microenvironment.
Tissue Specificity TGF-beta 1 expression is almost ubiquitous in all tissue types in concert with the wide-range of functions in wound healing, developmental cell patterning, regulation of epithelial and endothelial tissues, angiogenesis, bone remodeling, neuroprotection, and inflammatory regulation.
Cellular Localization Secreted protein as both a latent protein complex in the extracellular matrix and activated mature protein.
Involvement In Disease TGF-beta 1 plays an important role as a cancer tumor suppressor, however, dysregulation of TGF-beta 1 can promote tumor growth, metastasis and immune evasion that is associated with various cancers, including breast cancer, colorectal cancer, pancreatic cancer, and prostate cancer. Dysregulated TGF-β1 has been implicated in autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, multiple sclerosis, and inflammatory bowel diseases.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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