Recombinant Human Neurotrophin 3/NT-3 Protein, Active

Beta LifeScience SKU/CAT #: BEP-0057

Recombinant Human Neurotrophin 3/NT-3 Protein, Active

Beta LifeScience SKU/CAT #: BEP-0057
/
Size

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Product Overview

Product Name Recombinant Human Neurotrophin 3/NT-3 Protein
Target Symbol NTF3
Alternative Names Neurotrophin 3, NT3, NTF3, Nerve Growth Factor 2, NGF2, HDNF
Accession Number P20783
Expression System E. coli.
Molecular Weight 13.8 kDa (predicted)
Sequence Tyr149-Thr257
Purity Determined by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
Activity Determined by in-house activity assay
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level Available Upon Request
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function Neurotrophin 3 (NT-3) belongs to the neurotrophin family and primarily functions as a growth factor that promotes the survival, growth, and differentiation of neurons and oligodendrocytes. NT-3 stimulates the growth and branching of axons and dendrites. This process is crucial for the establishment of neuronal circuits during development and for the regeneration of neural connections after injury. NT-3 enhances neuroprotection and regulates synaptic plasticity in mature neurons. Outside of the CNS, NT-3 promotes differentiation and survival of peripheral, motor, and sensory neurons. NT-3 primarily binds to TrkC as the high affinity signaling receptor, but association with TrkB and the low-affinity p75-NTR has been reported. In cell culture, NT-3 has been used to maintain neuronal cells in culture, the generation of peripheral neurons and oligodendrocyte precursors. It has also been used to differentiate human pluripotent stem cells (hPSCs) to glutamatergic and GABAergic neurons and the culture of 3-D organoids.
Tissue Specificity NT-3 expression has been identified in brain (cerebral cortex, hippocampus, cerebellum), spinal cord, peripheral nerves, ganglia, and to a lesser extent in skin, skeletal muscles, heart, kidney, and liver.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of NT-3 is associated with Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, diabetic neuropathy, Charcot-Marie-Tooth disease, depression, anxiety, amyotrophic lateral sclerosis (ALS), and muscular dystrophies.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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