CD47 Protein: Target Overview, Research Applications, and Selection Guide
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Cluster of Differentiation 47 (CD47) is a widely expressed cell surface transmembrane glycoprotein belonging to the immunoglobulin superfamily. It functions as a key innate immune checkpoint, delivering a “don’t eat me” signal through interaction with Signal Regulatory Protein Alpha (SIRPα) on myeloid cells.
As a cell-surface ligand, CD47 inhibits macrophage-mediated phagocytosis and plays a central role in immune evasion, cell adhesion, and signaling regulation, particularly in cancer and inflammatory contexts.
CD47 Target Overview
CD47 is a plasma membrane–localized immune checkpoint protein that regulates phagocytosis through interaction with SIRPα on myeloid cells. It primarily functions in:
- Inhibition of macrophage-mediated phagocytosis
- Cell adhesion and migration
- Immune signaling regulation
- Tumor immune evasion
A key function is the CD47–SIRPα interaction, which activates SHP-1/2–mediated inhibitory signaling to suppress phagocytosis. CD47 exists in multiple splice isoforms that may differ in signaling properties.
CD47 Target Information
Target Name: CD47
Synonyms: Integrin-associated protein (IAP); MER6; OA3; Leukocyte surface antigen CD47
UniProt ID: Q08722
Target Class: Cell surface glycoprotein; immune checkpoint ligand; immunoglobulin superfamily protein
CD47 Mechanism of Action
CD47 on tumor cells binds SIRPα on macrophages to deliver a “don’t eat me” signal, activating SHP-1/2–mediated inhibitory pathways that suppress phagocytosis. Blocking the CD47–SIRPα interaction restores macrophage activity and promotes tumor cell clearance.
Key Research Applications of CD47 Protein
CD47 is best suited for cell-based, binding, and immuno-oncology studies, rather than enzyme-focused workflows.
1. Immune Checkpoint & Phagocytosis Studies
- CD47–SIRPα interaction analysis
- Macrophage phagocytosis assays
- Immune evasion mechanism studies
2. Antibody & Therapeutic Development
- Screening and characterization of anti-CD47 antibodies
- Epitope mapping and binding validation
- ADC and bispecific antibody development
3. Binding & Inhibition Assays
- CD47–SIRPα binding assays
- Inhibitor screening and IC₅₀ determination
- Competitive binding and blocking studies
4. Cell-Based Functional Assays
- Phagocytosis assays (co-culture systems)
- Reporter assays (immune activation pathways)
- Cytokine release studies
5. Cancer Research
- CD47 expression profiling in tumors
- Role in tumor immune escape
- Combination immunotherapy studies
6. Biomarker Development
- IHC / Flow cytometry detection
- ELISA-based quantification
- Patient stratification and prognosis analysis
CD47 Experimental Workflows and Use Cases
CD47 proteins are most effective in binding and cell-based workflows:
- Binding assays – CD47–SIRPα interaction characterization
- Blocking assays – antibody or inhibitor validation
- Phagocytosis assays – functional immune response evaluation
- Antibody development – epitope mapping and affinity testing
- Assay development – ELISA, flow cytometry, and cell-based assays
Because CD47 is a membrane protein, it is not suitable for enzyme activity assays, but highly suitable for cell-surface interaction and immune checkpoint studies.
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