Recombinant Human Interleukin-2 (IL2) Protein (GST)

Name: Recombinant Human Interleukin-2 (IL2) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-10775P
Price: 299.0 USD
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) IL2.

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Product Overview

Description	Recombinant Human Interleukin-2 (IL2) Protein (GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P60568
Target Symbol	IL2
Synonyms	Aldesleukin; IL 2; IL-2; IL2; IL2_HUMAN; Interleukin 2; Interleukin-2; interleukin2; Involved in regulation of T cell clonal expansion; Lymphokine; OTTHUMP00000164090; POIL2; T Cell Growth Factor; T-cell growth factor; TCGF
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT
Expression Range	21-153aa
Protein Length	Full Length of Mature Protein
Mol. Weight	42.4kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Subcellular Location	Secreted.
Protein Families	IL-2 family
Database References	HGNC: 6001 OMIM: 147680 KEGG: hsa:3558 STRING: 9606.ENSP00000226730 UniGene: PMID: 30072687 This study emphasized on the bone marrow and blood plasma levels of IL-2 in aplastic anaemia and their relationship with disease severity. The results indicate towards the fact that IL-2 may have an important association with the marrow failure of AAA patients and thus can help in disease development. Further study is necessary for better understanding. PMID: 30139310 The present study demonstrated no association between IL-2 (T-330G), IL-16 (T-295C), and IL-17 (A-7383G) genotypes and CP in an Iranian population. PMID: 29400002 In contrast with some reports involving the correlation between polymorphisms of the TGF-beta1 and IL-2 genes and inhibitor development in the world, no statistically significant differences in analysis of the alleles and genotypes for TGF-beta and IL-2 genes were found between the inhibitor and non-inhibitor Iranian patients PMID: 29993342 The current study highlights the possible involvement of the IL-2-330T/G Single Nucleotide Polymorphism in susceptibility to B-Cell Non-Hodgkin Lymphoma . Moreover, IL-10-1082A/G is not a molecular susceptibility marker for B-Cell Non-Hodgkin Lymphoma in Egyptians. PMID: 28713071 interleukin-2 (IL-2) is a non-pancreatic autoimmune target in type 1 diabetes PMID: 27708334 The IL-2 AC genotype and C allele of IL-2 (-330A>C) gene polymorphisms could be potential protective factors and might reduce the risk of oral cancer in Indian population. PMID: 29664031 Data show that GIF (ORF117 ) serves as a competitive decoy receptor by leveraging binding hotspots underlying the cognate receptor interactions of granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin-2 (IL-2). PMID: 27819269 CD4(+) T cells showed the greatest increase (threefold) in ORMDL3 expression in individuals carrying the asthma-risk alleles, where ORMDL3 negatively regulated interleukin-2 production. The asthma-risk variants rs4065275 and rs12936231 switched CTCF-binding sites in the 17q21 locus. PMID: 27848966 The main mechanism of elimination of mesenchymal stromal cells is cytotoxicity of NK cells that depended on IL-2 production. PMID: 29313232 Uremia patients receiving maintenance hemodialysis with hospitalacquired infection had increased serum inflammatory factors and high throughput hemodialysis significantly decreased CRP, IL2 and TNFalpha levels in the serum, suggesting that high throughput hemodialysis may be beneficial for the prevention of the infections in uremia patients. PMID: 29257244 The simultaneous delivery of multiple proinflammatory payloads to the cancer site conferred protective immunity against subsequent tumor challenges. A fully human homolog of IL2-F8-TNF(mut), which retained selectivity similar to its murine counterpart when tested on human material, may open new clinical applications for the immunotherapy of cancer. PMID: 28716814 Study found that in SLE patients lymphocyte production of IL-2 did not decrease when compared with that of normal subjects. PMID: 29911835 Peripheral blood Tregs failed to effectively utilize IL-2 and had relatively little STAT5 phosphorylation in active ankylosing spondylitis. PMID: 27901054 correction for individual cytokine expression levels revealed qualitative differences of cytokine profiles characterized by significantly increased TNFalpha and decreased IL-2-expressing T-cell proportions in long-term type-1-diabetes patients. PMID: 28377612 CD45 is a regulator of IL-2 synergy in the NKG2D-mediated activation of immature human NK cells PMID: 28655861 genetic polymorphism is associated with increased susceptibility to chronic spontaneous urticaria in Iran PMID: 28159384 PLX4032, a selective BRAF-i, has no inhibitory effect either on NK cell proliferation in response to cytokines IL-2 or IL-15 PMID: 27563819 Results suggest that IL-6 and IL-2 were influenced non-specifically by the presence of a mental disorder and by demographic variables of gender, ethnicity and BMI. Implications of these findings are discussed, as well as possible long-term impact of the identified interleukin differences on immunologic, inflammatory, neuropsychiatric and other systems. PMID: 28486207 possible associations between the IL-2 polymorphisms +114 T>G (rs2069763) and -330 T>G (rs2069762) and the development of gastric cancer; these associations were then correlated with the presence of H. pylori; results show that, among patients with H. pylori infection, the -330 GG and +114 TT genotypes are significantly associated with a high risk of developing gastric cancer, as is the -330G/+114T haplotype PMID: 28458166 Overrepresentation of certain alleles, genotypes and haplotypes in IL-2 gene in febrile seizure patients could predispose individuals to this disease PMID: 28843235 Data reported that let-7i upregulates IL-2 expression by targeting the promoter TATA-box region, which functions as a positive regulator. In HIV-1 infection, the expression of let-7i in CD4(+) T cells is decreased by attenuating its promoter activity. The reduced let-7i miRNA expression led to a decline in IL-2 levels which contributes to CD4(+) T cells death. PMID: 27145859 IL-2 and IL-6 work together to enhance influenza-specific CD8 T cell generation responding to live influenza virus in aged mice and humans PMID: 27322555 the aim of the current review article is to determine the roles of IL-2 IL-2/IL-2R interaction in polyoma BK virus reactivation and PBK associated nephropathy complications. PMID: 27718431 Modulation of IL-2, IL-4, IFN-gamma and/or TNF-alpha levels, or inhibitors of Erk1/2 or S6K1 may be a new approach to prevent BAFF-induced aggressive B-cell malignancies. PMID: 27235588 Our results show that the frequency of Tregs is altered in a large cohort of long-term T1D patients, a profound decrease in CD25 expression and altered IL-2 signaling are typical features of Tregs populations in long-term diabetic patients and their relatives. PMID: 27560779 a role for circulating IL-2 in liver dysfunction and propose that a combined assessment of AST/ALT in conjunction with IL-2 at the early stages of symptomatic DENV infection may be useful to predict the severe forms of dengue. PMID: 27155816 Lymphocytes incubated in the presence of IL-2 lose the capacity for chemotaxis but acquire antitumor activity PMID: 28429264 the central biological role of the novel IL-2-R/Lck/PLCgamma/PKCtheta;/alphaPIX/Rac1/PYGM signalling pathway is directly related to the control of fundamental cellular processes such as T cell migration and proliferation. PMID: 27519475 F42K can circumvent the expansion and negative immunoregulatory effects of highly suppressive ICOS+ Tregs, while promoting NK cell expansion and function. F42K also induces a unique gene expression profile and does not activate many IL2-induced genes, although it has the capacity to activate NK cells and NK cell-associated activation genes, costimulatory molecules, and NK-mediated cytolytic function PMID: 27697858 Chimeric fusion protein of interleukin 2 (IL2) and its receptor interleukin 2 receptor, beta protein (IL2Rbeta) enhances antitumor activity of natural killer NK92 cells. PMID: 28916655 Il2 was not a useful discriminative markers for active tuberculosis among pulmonary tuberculosis suspects. PMID: 27450011 IL-2 and IL-10 could work synergistically to improve the survival, proliferation, and cytotoxicity of activated CD8(+) T cells, an effect suppressible by CD4(+)CD25(+) Treg cells PMID: 28274688 results show that IL-23 accounts for the main aspects of human and murine lupus including the expansion of double negative T cells, decreased IL-2, and increased IL-17 production PMID: 28646040 Report efficient retroviral vector transduction of primary human natural killer cells that were stimulated by a combination of IL-2 and engineered K562 cells expressing membrane-bound IL-21 for cancer immunotherapy. PMID: 28802832 upon ligation of the T-cell antigen receptor (TCR), the TCR associates with and transactivates CXCR4 via phosphorylation of S339-CXCR4 in order to activate a PREX1-Rac1-signaling pathway that stabilizes interleukin-2(IL-2), IL-4, and IL-10 messenger RNA (mRNA) transcripts. PMID: 28694325 IL2 induces disruption of adherens junctions, concomitant with cytoskeletal reorganization, ultimately leading to increased endothelial cell permeability. PMID: 27601468 findings extend understanding of the differential modes of action between IL-2 and IL-15, and highlight how, although sharing the CD122/CD132 receptor, they elicit such different immune actions PMID: 28507024 DNA-PKcs is a potent regulator of IL-2 production in T lymphocytes. PMID: 28750002 Yeast-expressed human IL-2 fusion toxin effectively targetedCD25(+) cutaneous T cell lymphoma. PMID: 28551309 study provides evidence that membrane-coated microvesicles released by apoptotic neutrophils suppress a subset of resting Th cells by downregulating IL-2 and IL-2R expression PMID: 28295230 In T1D, low IL-2 responsiveness was most pronounced in memory T effector cells. Reduced IL-2 responses in memory T effector cells were not rescued by resting, remained lower after activation and proliferation, and were absent in type 2 diabetes. PMID: 28645874 the elevated level of IL-2+ and IL-21+ T cells and a positive correlation between IL-21+ cells with clinical activity index in ulcerative colitis patients may contribute to the pathogenesis of disease. PMID: 28685527 analysis of the contribution of IL2Rgamma to the dynamic formation of IL2-IL2R complexes PMID: 27195783 lack of local IL-2 enhances regulatory T-cell susceptibility to Fas-mediated apoptosis induced by epithelial cells PMID: 26928938 The IL-2 rs2069762 G allele appeared to be a protective mutation against aplastic anemia, but no significant differences were found in other four IL-2 and Il-8 SNPs studied. PMID: 28268223 Pristimerin inhibits IL-2 induced T cell activation and generation of lymphokine-activated killer cells by disrupting multiple cell signaling pathways induced by IL-2. PMID: 28471123 IL-2 or induced killer cells combination therapy was efficacious in treating NSCLC and improved overall survival. Further analysis of trials having adequate information and data need to be done to confirm these findings. PMID: 27748271 Treatment of memory CD4 T cells with the concentration of kynurenine found in plasma inhibited IL-2 signaling through the production of reactive oxygen species. PMID: 27356894 Ochratoxin A mediates MAPK activation, modulates IL-2 and TNF-alpha mRNA expression and induces apoptosis by mitochondria-dependent and mitochondria-independent pathways in human CD4 positive T-cell lymphoma cell line. PMID: 27193732

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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