Recombinant Human Epoxide Hydrolase 1 (EPHX1) Protein (His-SUMO)

Name: Recombinant Human Epoxide Hydrolase 1 (EPHX1) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-03161P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Epoxide Hydrolase 1 (EPHX1) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P07099
Target Symbol	EPHX1
Synonyms	EPHX 1; EPHX; Ephx1; EPOX; Epoxide hydratase; Epoxide hydrolase 1; Epoxide hydrolase 1 microsomal (xenobiotic); Epoxide hydroxylase 1; Epoxide hydroxylase 1 microsomal (xenobiotic); HYEP_HUMAN; HYL1; MEH; Microsomal epoxide hydrolase
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MWLEILLTSVLGFAIYWFISRDKEETLPLEDGWWGPGTRSAAREDDSIRPFKVETSDEEIHDLHQRIDKFRFTPPLEDSCFHYGFNSNYLKKVISYWRNEFDWKKQVEILNRYPHFKTKIEGLDIHFIHVKPPQLPAGHTPKPLLMVHGWPGSFYEFYKIIPLLTDPKNHGLSDEHVFEVICPSIPGYGFSEASSKKGFNSVATARIFYKLMLRLGFQEFYIQGGDWGSLICTNMAQLVPSHVKGLHLNMALVLSNFSTLTLLLGQRFGRFLGLTERDVELLYPVKEKVFYSLMRESGYMHIQCTKPDTVGSALNDSPVGLAAYILEKFSTWTNTEFRYLEDGGLERKFSLDDLLTNVMLYWTTGTIISSQRFYKENLGQGWMTQKHERMKVYVPTGFSAFPFELLHTPEKWVRFKYPKLISYSYMVRGGHFAAFEEPELLAQDIRKFLSVLERQ
Expression Range	1-455aa
Protein Length	Full Length
Mol. Weight	68.9kDa
Research Area	Metabolism
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. Plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids. Metabolizes the abundant endocannabinoid 2-arachidonoylglycerol (2-AG) to free arachidonic acid (AA) and glycerol.
Subcellular Location	Microsome membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein.
Protein Families	Peptidase S33 family
Database References	HGNC: 3401 OMIM: 132810 KEGG: hsa:2052 STRING: 9606.ENSP00000272167 UniGene: PMID: 27256986 EPHX1 variants were significantly associated with higher metabolic ratio of carbamazepine PMID: 27276192 Single nucleotide polymorphisms in EPHX1, GSTP1, SERPINE2, and TGFB1 contributing to the quantitative traits of chronic obstructive pulmonary disease in Chinese Han population. PMID: 27193053 This study showed that polymorphisms of EPHX1 and VKORC1L1 could be determinants of stable warfarin doses. PMID: 29054760 The Tyr113His T/C variant of rs1051740 and very slow phenotype alters EPHX1, miR-26b-5p and miR-1207-5p expression, and contributes towards low blood iron levels and low birthweights. PMID: 28789952 Structural characterization of human microsomal epoxide hydrolase by combined homology modeling, molecular dynamics simulations, and molecular docking calculations has been reported. PMID: 28120429 Y113H polymorphism in EPHX1 gene contributed to increased susceptibility to COPD in the Kazakhstan population. PMID: 28464990 EPHX1 polymorphisms were not associated with sporadic colorectal neoplasms. PMID: 28018104 This study demonstrated that two SNPs might play roles in the process of nicotine metabolism and abstinence, rs1051740 being more important; and EPHX SNPs (rs1051740 and rs2234922) are associated with the effectiveness of Nicotine Replacement Therapy . PMID: 27783326 No association was found between EPHX1 and COPD; however, a minor effect of EPHX1 on COPD risk was not completely excluded. PMID: 27173271 The SCN1A IVS5-91G>A SNP is associated with susceptibility to epilepsy. SNPs in EPHX1 gene are influencing CBZ metabolism and disposition PMID: 26555147 In conclusion, GSTs, EPHX1, and XPD are potential genetic factors for arsenic-induced skin cancers. The roles of these genes for arsenic-induced skin carcinogenesis need to be further evaluated. PMID: 26295053 the EPHX1 Tyr113His polymorphism may be a risk factor for lung cancer in Asians, whereas it may be a decreased risk factor among Caucasians. However, this polymorphism was not found to be associated with breast cancer. PMID: 25222243 To be poly(ADP-ribose)polymerase-1 (PARP-1) bound to the EPHX1 proximal promoter. PMID: 25992604 Polymorphism in the EPHX1 gene may have a significant role in differential responses to treatment with N-acetylcysteine in patients with COPD. PMID: 25999707 This review and meta-analysis suggests that EPHX1 Tyr113His polymorphism may be a risk factor for Head and Neck Cancer, while the EPHX1 His139Arg polymorphism has no association with Head and Neck Cancer risk. PMID: 25923690 Studies suggest that the presence of microsomal epoxide hydrolase 1 (EPHX1) single nucleotide polymorphisms (SNPs) may significantly affect the risk of lung, upper aerodigestive tract, breast, bladder, and ovarian carcinomas. PMID: 26216302 the genetic associations exist between mEH polymorphisms and lung cancer susceptibility in Asian populations PMID: 25312477 These results demonstrate that 2-AG is an endogenous substrate for EPHX1, a potential role of EPHX1 in the endocannabinoid signaling and a new AA biosynthetic pathway. PMID: 24958911 EPHX1 rs2292566 polymorphism may affect the maintenance dose of warfarin in Caucasians. PMID: 25629049 His/His genotype of EPHX1 Tyr113His polymorphism is a risk factor for developing caner for Asian and mixed population, while no evidence was found for the association between the EPHX1 His139Arg polymorphism and increased cancer risk. PMID: 25261893 EPHX1 Tyr113His and His139Arg polymorphism are not associated with esophageal cancer risk. PMID: 25714851 This meta-analysis suggests that EPHX1 Tyr113His polymorphism contributes to HCC risk. PMID: 26065263 No clear effect is evident for EPHX1 polymorphisms for different radon concentrations on the risk of lung cancer. PMID: 24852519 Review/Meta-analysis: suggest p.Tyr113His and p.His139Arg polymorphisms in EPHX1 may not be associated with esophageal cancer development. PMID: 24803829 This study provides direct evidence that methylation plays an important role in PCOS and demonstrates a novel role for EPHX1 in female reproduction. PMID: 24505354 Meta-analysis showed that the Tyr113His polymorphism was a stronger power trend towards risk for esophageal cancer using a recessive model PMID: 24615030 The association of EPHX1 polymorphism with the HELLP syndrome and eclampsia may hint to EPHX being a further key player in the pathogenesis of preeclampsia. PMID: 24013430 Results suggest that Exons 3 and 4 polymorphisms of the mEH gene may contribute to lung cancer susceptibility through disturbed antioxidant balance. PMID: 23928928 The C allele and C-G diplotype of EPHX1 may play important roles in increasing the risk of CBZ-SJS/toxic epidermal necrolysis development of epilepsy in Chinese Han patients. PMID: 24861996 Chinese epilepsy patients with variant EPHX1 c.416A>G genotype have higher plasma carbamazepine concentrations compared to those with the wild type genotype. PMID: 24125961 No evidence of an association of EPHX1 Tyr113His or His139Arg polymorphisms with risk for development of esophageal cancer. [Meta-analysis] PMID: 24222229 EPHX1 genetic polymorphisms were not associated with the risk of HCC. PMID: 23955801 The study showed that microsomal epoxide hydrolase exon 3 113Tyr-139Arg was associated with gastric cancer in presence of H. pylori, though in its absence, it appeared to be protective. PMID: 23580125 Sp1 and Sp3 are functionally involved as transcriptional integrators regulating the basal expression of the derived microsomal epoxide hydrolase E1b variant transcript. PMID: 24315822 Genotype frequencies for EPHX1 polymorphisms did not show any correlation with lymphoma. PMID: 23651475 This meta-analysis suggests that EPHX1 His139Arg polymorphism is associated with decreased risk of esophageal cancer in Caucasians. PMID: 23681797 There is substantial evidence that mEH polymorphisms interact synergistically with other genes and the environment to modulate risk of HCC PMID: 23451147 findings suggest that benzene exposure may be associated with hypermethylation in ERCC3, and that genetic variants in EPHX1 may play an important role in epigenetic changes and hematotoxicity among benzene-exposed workers PMID: 23797950 This is the first report that examined HIF1A polymorphisms in chronic obstructive pulmonary disease and demonstrated a possible role of HIF-1alpha in COPD, as well as GSTP1 and EPHX1. PMID: 21651746 EPHX1 transcript, termed E1-b', drives EPHX1 expression primarily in the ovary. PMID: 23564882 EPHX1 gene polymorphisms and haplotypes are associated with an increased risk for alcoholism in the Kota Indian population PMID: 22257321 minor Tyr113 allele of mEPHX1 polymorphism had a higher risk of type 2 diabetes mellitus PMID: 22555758 Microsomal epoxide hydrolase 1 T113C polymorphism is associated with lung cancer. PMID: 23378225 the maternal EPHX1 rs1051740 genotype (RR = 3.26, P = .01) was associated with medulloblastoma risk. PMID: 22994552 Meta-analyses of available data supported the concept of EPHX1 A139G polymorphism as a genetic susceptibility factor for lung cancer PMID: 23055191 EPHX1 gene polymorphisms and haplotypes were not involved in the susceptibility to oral cancer in South Indian subjects. PMID: 21453055 EPHX1 Tyr113His polymorphism may be not associated with CRC development PMID: 22928041 Study analyzed the association between four SNPs in the EPHX1 and EPHX2 and the risk of oligozoospermia and asthenospermia; rs1051740, rs2234922 and rs751141 were not associated with oligozoospermia and asthenospermia and rs1042064 was a protective factor in idiopathic male infertility. PMID: 22986331 microsomal epoxide hydrolase polymorphism is associated with chromosomal instability of 1,3-butadiene exposed workers. PMID: 22156006

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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