Native Human Eosinophil Peroxidase (EPO) Protein

Name: Native Human Eosinophil Peroxidase (EPO) Protein
Brand: Beta LifeScience
SKU: BAR-0011P
Availability: InStock

Collections: All products, Native proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	Native Human Eosinophil Peroxidase (EPO) is a highly purified, enzymatically active hemoprotein isolated from human eosinophils. This preparation captures the complete, native heterodimeric structure of the enzyme, preserving its potent peroxidatic activity and unique substrate profile. As a key effector molecule in eosinophil function, it is an indispensable tool for research in immunology, inflammation, and host-pathogen interactions.
Source	Human Whole Blood
Form	Lyophilized Powder
Buffer	Lyophilized from 20 mM NaHPO4, pH 7.5, and 150 mM NaCI
Activity	≥ 180 units/mg protein after lyophilization. One unit of activity is defined as the amount of enzyme that will decompose 1 μmol of H2O2 per minute at 25 °C, pH 6.0
Purity	Greater than 95% as determined by SDS-PAGE.
Molecular Weight	70.5kDa
Protein Determination	Based on a molar extinction coefficient of @ 413 mM of 110,000 M-1 cm -1
Storage/Handling	≤ -20 °C

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.