Rabbit anti-Human H2AFX Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00589A

Western Blot | Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate | All lanes: H2AFX antibody at 1.8μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 16 kDa | Observed band size: 16 kDa

Immunofluorescence staining of Hela cells with Phospho-H2AFX (S139) Antibody at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFX (Phospho-H2AFX (S139) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Rabbit anti-Human H2AFX Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00589A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Product Title | Rabbit anti-Human H2AFX Polyclonal Antibody |
Description | The antibody against H2AFX was raised in rabbit using the Peptide sequence around site of Phospho-Ser (139) derived from Human Histone H2AX as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IF, ChIP. |
Uniprot Id - Alink | P16104 |
Host Species | Rabbit |
Reactivity | Human |
Target Name | H2AFX |
Target Synonyms | H2A histone family member X antibody; H2A histone family member X antibody; H2A.FX antibody; H2A.X antibody; H2a/x antibody; H2AFX antibody; H2AX antibody; H2AX_HUMAN antibody; Histone H2A.X antibody |
Immunogen Description | Peptide sequence around site of Phospho-Ser (139) derived from Human Histone H2AX |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Polyclonal |
Isotype | IgG |
Purification Method | Antigen affinity purified |
Buffer | 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Form | Liquid |
Application | ELISA, WB, IF, ChIP |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |