Products H2afx

7 products

Filters

    7 products
    Western Blot | Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate | All lanes: H2AFX antibody at 1.64μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 16 kDa | Observed band size: 16 kDa
    IHC image of H2AFX (Ab-139) Antibody diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    Rabbit anti-Human H2AFX Polyclonal Antibody
    CAT#: BLC-00587A
    Western Blot | Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate | All lanes: H2AFX antibody at 1.8μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 16 kDa | Observed band size: 16 kDa
    Immunofluorescence staining of Hela cells with Phospho-H2AFX (S139) Antibody at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    Rabbit anti-Human H2AFX Polyclonal Antibody
    CAT#: BLC-00589A
    Western blot analysis of extracts from HepG2 and 293 cells using Histone H2A.X(Ab-139) Antibody.
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H2A.X(Ab-139) Antibody(left) or the same antibody preincubated with blocking peptide(right).
    Rabbit anti-Human H2AFX Polyclonal Antibody
    CAT#: BLC-05496A
    Western blot analysis of extracts from HT29 cells untreated(lane 1) or treated with UV(lane 2) using Histone H2A.X(Phospho-Ser139) Antibody.
    Immunofluorescence staining of methanol-fixed Hela cells using Histone H2A.X(Phospho-Ser139) Antibody.
    Rabbit anti-Human H2AFX Polyclonal Antibody
    CAT#: BLC-05565A
    Greater than 90% as determined by SDS-PAGE.
    Recombinant Human Histone H2A.X (H2AFX) Protein (GST)
    • Molecule: H2AX;
    • Species: Human;
    • Expression system: E.coli;
    • Tag: GST;
    • Sequence: Full Length Protein;
    CAT#: BLC-03481P

    Online Inquiry
    Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) H2AFX,H2AX .
    Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) H2AFX,H2AX .
    Recombinant Human Histone H2Ax (H2AFX) Protein (His)
    • Molecule: H2AX;
    • Species: Human;
    • Expression system: E.coli;
    • Tag: His;
    • Sequence: Protein Fragment;
    CAT#: BLC-08144P

    Online Inquiry
    Greater than 85% as determined by SDS-PAGE.
    Recombinant Human Histone H2Ax (H2AFX) Protein (His-GST)
    • Molecule: H2AX;
    • Species: Human;
    • Expression system: E.coli;
    • Tag: His-GST;
    • Sequence: Full Length Protein;
    CAT#: BLC-00030P

    Online Inquiry