Rabbit anti-Human H2AFX Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00587A

Western Blot | Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate | All lanes: H2AFX antibody at 1.64μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 16 kDa | Observed band size: 16 kDa

IHC image of H2AFX (Ab-139) Antibody diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of H2AFX (Ab-139) Antibody diluted at 1:50 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Rabbit anti-Human H2AFX Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00587A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Product Title | Rabbit anti-Human H2AFX Polyclonal Antibody |
Description | The antibody against H2AFX was raised in rabbit using the Peptide sequence around site of Ser (139) derived from Human Histone H2AX as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IHC, IF, ChIP. |
Uniprot Id - Alink | P16104 |
Host Species | Rabbit |
Reactivity | Human |
Target Name | H2AFX |
Target Synonyms | H2A histone family member X antibody; H2A histone family member X antibody; H2A.FX antibody; H2A.X antibody; H2a/x antibody; H2AFX antibody; H2AX antibody; H2AX_HUMAN antibody; Histone H2A.X antibody |
Immunogen Description | Peptide sequence around site of Ser (139) derived from Human Histone H2AX |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Polyclonal |
Isotype | IgG |
Purification Method | Antigen affinity purified |
Buffer | 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Form | Liquid |
Application | ELISA, WB, IHC, IF, ChIP |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |