Products Polr2a

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    3 products
    Western Blot | Positive WB detected in Hela whole cell lysate,A549 whole cell lysate,293 whole cell lysate | All lanes Phospho-POLR2A antibody at 1.02μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 270 KDa | Observed band size: 270 KDa
    Phospho-POLR2A (S2) Recombinant Monoclonal Antibody
    CAT#: BLC-06483A
    Western Blot | Positive WB detected in Hela whole cell lysate,A549 whole cell lysate,293 whole cell lysate | All lanes Phospho-POLR2A antibody at 0.75μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 270 KDa | Observed band size: 270 KDa
    Immunocytochemistry analysis of Phospho-POLR2A (S5) Recombinant Monoclonal Antibody diluted at 1:75 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    Phospho-POLR2A (S5) Recombinant Monoclonal Antibody
    CAT#: BLC-06482A
    IHC image of the antibody diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    IHC image of POLR2A Antibody diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    Rabbit anti-Human POLR2A Polyclonal Antibody
    CAT#: BLC-00006A