Phospho-POLR2A (S5) Recombinant Monoclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-06482A

Western Blot | Positive WB detected in Hela whole cell lysate,A549 whole cell lysate,293 whole cell lysate | All lanes Phospho-POLR2A antibody at 0.75μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 270 KDa | Observed band size: 270 KDa

Immunocytochemistry analysis of Phospho-POLR2A (S5) Recombinant Monoclonal Antibody diluted at 1:75 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with Phospho-POLR2A (S5) Recombinant Monoclonal Antibody at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Phospho-POLR2A (S5) Recombinant Monoclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-06482A
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Product Overview
Product Title | Phospho-POLR2A (S5) Recombinant Monoclonal Antibody |
Description | The recombinant antibody against POLR2A was produced using a synthesized peptide derived from Human Phospho-POLR2A (S5) as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IHC, IF. |
Uniprot Id - Alink | P24928 |
Reactivity | Human |
Target Name | POLR2A |
Target Synonyms | DNA directed RNA polymerase II A antibody; DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit antibody; DNA-directed RNA polymerase II subunit A antibody; DNA-directed RNA polymerase II subunit RPB1 antibody; DNA-directed RNA polymerase III largest subunit antibody; hRPB220 antibody; hsRPB1 antibody; POLR2 antibody; Polr2a antibody; POLRA antibody; Polymerase (RNA) II (DNA directed) polypeptide A 220kDa antibody; Polymerase (RNA) II (DNA directed) polypeptide A antibody; RNA polymerase II subunit B1 antibody; RNA-directed RNA polymerase II subunit RPB1 antibody; RPB1 antibody; RPB1_HUMAN antibody; RPBh1 antibody; RpIILS antibody; RPO2 antibody; RPOL2 antibody |
Immunogen Description | A synthesized peptide derived from Human Phospho-POLR2A (S5) |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Monoclonal |
Isotype | Rabbit IgG |
Purification Method | Affinity-chromatography purified |
Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Form | Liquid |
Application | ELISA, WB, IHC, IF |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |