Recombinant Mouse SEMA4D Protein (C-Fc)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Mouse Semaphorin 4D is produced by our Mammalian expression system and the target gene encoding Phe24-Met711 is expressed with a human IgG1 Fc tag at the C-terminus.
Accession	O09126
Synonym	SEMA4D; Semaphorin-4D; M-Sema G; Semaphorin-C-like 2; Semaphorin-J; Sema J; CD100; Semacl2; Semaj
Gene Background	SEMA4D is a member of the semaphorin family,contains one Ig-like C2-type domain, one PSI domain and one Sema domain. SEMA4D is strongly expressed in lymphoid tissues, especially in the thymus, as well as in the nervous tissues. However, SEMA4D is expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver. SEMA4D is a cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. SEMA4D is involved in a number of fundamental biological processes such as promoting reorganization of the actin cytoskeleton, the migration of cerebellar granule cells and of endothelial cells and signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Not only these, it plays a role in axonal growth cone guidance in the developing central nervous system. Semaphorin-4D / SEMA4D may play a functional role in the immune system, as well as in the nervous system. It could induce B-cells to aggregate and improves their viability (in vitro). SEMA4D is involved in regulating dendrite and axon branching and morphogenesis and promoting interaction with PLXNB1 mediates activation of RHOA.
Molecular Mass	103.4 KDa
Apmol Mass	110-130 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.
Subcellular Location	Cell membrane; Single-pass type I membrane protein.
Protein Families	Semaphorin family
Database References	KEGG: mmu:20354 STRING: 10090.ENSMUSP00000021900 UniGene: Mm.33903
Tissue Specificity	Strongly expressed in lymphoid tissues, especially in the thymus, as well as in the nervous tissues. Expressed in neurons and glia in the developing hippocampus.

Gene Functions References

These results reveal a novel signaling network, the Sema4D-RhoA-Akt signal cascade, that coordinates cellular function and morphology and highlights the importance of specific spatiotemporally restricted components of a signaling pathway in the regulation of ameloblast differentiation. PMID: 27218883
our findings revealed for the first time that Sema4D positively regulated both the adaptive and innate immune responses in contact hypersensitivity PMID: 27614265
SEMA4D-/- neutrophils show impaired NET formation when in contact with endothelial cells. PMID: 28416516
Absence of Sema4D improves oligodendrocyte recovery after cerebral ischemia/reperfusion injury in mice PMID: 26752319
Sema4D-deficiency affected ischemia-induced microglial activation in morphology, iNOS expression, and proliferation, and this deficiency most likely resulted in moderation of cytotoxicity and locomotor behavioral abnormality after cerebral ischemia PMID: 26202989
The addition of recombinant Sema4D to Sema4D/ vaginal epithelial cells in culture significantly enhanced apoptosis of the vaginal epithelial cells. PMID: 25351707
Sema4D may be an essential apoptosis-inducing ligand that acts downstream of estrogen action in vaginal epithelium during pubertal tissue remodeling. PMID: 24841081
therapeutic effects via bone-targeting systems featuring interference of Sema4d are able to partly counteract alveolar bone loss caused by osteoporosis. PMID: 25252878
CD100-mediated signals are critical for effective activation of gammadelta IEL to produce growth factors, including KGF-1, that are required for healing of the colon epithelium during colitis. PMID: 23695512
Deletion of plexin-B1, the high affinity receptor for semaphorin 4D, significantly (one-way ANOVA; p < 0.001) reduced thrombus weight (- 40%) in oxidative venous thrombosis. PMID: 23642655
Data indicate neuroimmune semaphorin 4D (Sema4D) as an important regulator of Th2-driven lung pathophysiology and as a potential target for a combinatory disease immunotherapy. PMID: 23911404
Sema 4D/CD100 is not only a ligand for plexin B1 but also a direct signaling receptor in mediating Sema 4D/CD100-plexin B's biological functions. PMID: 23262975
CD100 deficiency in vivo resulted in delayed repair of cutaneous wounds due to a disrupted gamma-delta T cell response to keratinocyte damage. PMID: 22902232
Sema4D may act as an intrinsic inhibitory regulator of oligodendrocyte differentiation by promoting apoptosis. PMID: 22198439
in vivo, Sema4D is an indirect regulator of bone resorption, which acts via its effect on reproductive function PMID: 22046317
Data show that osteoclasts express semaphorin 4D (Sema4D), previously shown to be an axon guidance molecule, which potently inhibits bone formation. PMID: 22019888
Studies identify Plxdc1, Sema4D, and neuropilin-1 as novel LRP1-regulated cell-signaling proteins. PMID: 20919742
Loss of Semaphorin 4D is associated with defect in collagen-induced platelet aggregation and an impaired vascular injury response. PMID: 20855865
Findings suggest that Sema4D is involved in the progression phase of atherosclerosis by accelerating intimal neovascularization, resulting in enhanced macrophage infiltration in atherosclerotic plaques. PMID: 20514420
Sema4D enhances inducible nitric oxide synthase (iNOS) and nitric oxide (NO) production in microglia. PMID: 20038643
Sema4D-plexin-B2 interactions regulate mechanisms underlying cell specification, differentiation, and migration during corticogenesis. PMID: 19948886
Sema4D is involved in oligodendrogenesis during development and during recovery from ischemic injury. PMID: 19472224
CD100, which enhances the maturation of DCs, is essential in the activation and differentiation of Ag-specific T cells. PMID: 12133937
Semaphorin 4D triggers invasive growth, a complex programme that includes cell dissociation, anchorage-independent growth and branching morphogenesis. PMID: 12198496
enhances the in vitro activation and differentiation of T cells and the in vivo generation of antigen-specific T cells PMID: 12374982
Sema4D is a novel inhibitory factor for axonal regeneration expressed in myelin PMID: 14534257
Sema4D may function as a neurite-outgrowth stimulating factor in embryonic sensory neurons. PMID: 15330859
Strength of B-cell antigen receptor signals is strictly tuned by the interaction of CD100 with CD72; this interaction is essential for maintaining immunological homeostasis as well as generating a proper immune response. PMID: 16113236
CD100 enhances pathogenetic humoral immune responses and promotes the activation of Antigen-Presenting Cells by up-regulating CD86 expression PMID: 16920982
proteolytic cleavage of Semaphorin 4D may provide a novel molecular mechanism by which membrane type 1-matrix metalloproteinase controls tumor-induced angiogenesis PMID: 17204469
semaphorin 4D has a role in platelet responses to vascular injury PMID: 17244710
In the ovarian follicle, the effect of Plexin-B1 is mediated by sema-4D. PMID: 17376242
Sema4D modulates T cell-APC interactions in allogeneic responses both in vivo and in vitro. PMID: 17950916
that tumor-associated macrophages (TAMs) are the main cells producing Sema4D within the tumor stroma and that their ability to produce Sema4D is critical for tumor angiogenesis and vessel maturation. PMID: 18559453
A functional role for CD100 antigen/Plxnb1 interactions in epithelial branching morphogenesis during organogenesis is reported. PMID: 18799546
loss of sema4D (semaphorin 4D) expression reduces the platelet hyperactivity otherwise found in dyslipidemia, and confers protection against the development of atherosclerosis PMID: 19390055
These results suggest that SHP2-mediated tyrosine dephosphorylation is an important step in Sema4D-induced axon repulsion. PMID: 19433062
these findings suggest a novel role of Sema4D/CD100 in the regulation of microglia activation providing a valuable neuroprotective tool PMID: 19467284
Mouse behavioral analyses revealed enhanced motor activity in Sema4D-deficient mice, suggesting the crucial involvement of Sema4D in the neurodevelopmental processes of the central structures mediating motor behavior in mice. PMID: 19534337

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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