tag |
GST |
host species |
Mouse |
accession |
NM_023913 |
synonym |
Ire1a; Ire1p; C85377; AI225830; Ire1alpha; 9030414B18Rik |
background |
ERN1 or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells (1). The activation of ERN1 through oligomerization expands the mechanistic repertoire of kinase-based signaling receptors. ERNI has a critical function in extraembryonic cells that is essential for fetal viability (2). |
description |
Recombinant mouse ERN1 (IRE1) (469-end) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods. |
source |
Sf9 insect cells |
AA sequence |
469a.a.-end |
molecular weight |
87 kDa |
purity |
For specific purity information on a given lot, see related COA. |
endotoxin |
< 1.0 EU per μg of the protein as determined by the LAL method |
bioactivity |
Active |
formulation |
Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
stability |
The recombinant protein is stable for up to 12 months at -70℃ |
usage |
For Research Use Only |
storage |
Recombinant Mouse ERN1 (IRE1) Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |