Recombinant Human pRB Protein (His Tag)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	NM_000321.
Synonym	OSRC; p105-Rb; pp110; pRb; RB.
Background	pRB, the product of retinoblastoma susceptibility gene RB-1, is one of the best-studied tumor suppressor gene products. At least two other pRB-related proteins, p107 and p130, have been identified and characterized. Mutations in RB-1 gene are often associated with the occurrence of various tumors (1-3). The activity of pRB is regulated through phosphorylation in a cell cycle-dependent manner. The hyperphosphorylated RB protein usually associates with the cell nucleus and binds transcription factors of the E2F family (4, 5). pRB represses transcription of its target genes, such as cdc2, cyclin A, and oncogene c-Myc, c-Fos through the binding with E2F factors and thereby regulating cell proliferation (6).
Description	Recombinant Human pRB Protein was expressed in Baculovirus-insect cell. This protein is fused with a His tag and purified using our unique purification methods.
Source	Baculovirus-insect cell
AA Sequence	TPRKTAATAA AAAAEPPAPP PPPPPEEDPE QDSGPEDLPL VRLEFEETEE PDFTALCQKL KIPDHVRERA WLTWEKVSSV DGVLGGYIQK KKELWGICIF IAAVDLDEMS FTFTELQKNI EISVHKFFNL LKEIDTSTKV DNAMSRLLKK YDVLFALFSK LERTCELIYL TQPSSSISTE INSALVLKVS WITFLLAKGE VLQMEDDLVI SFQLMLCVLD YFIKLSPPML LKEPYKTAVI PINGSPRTPR RGQNRSARIA KQLENDTRII EVLCKEHECN IDEVKNVYFK NFIPFMNSLG LVTSNGLPEV ENLSKRYEEI YLKNKDLDAR LFLDHDKTLQ TDSIDSFETQ RTPRKSNLDE EVNVIPPHTP VRTVMNTIQQ LMMILNSASD QPSENLISYF NNCTVNPKES ILKRVKDIGY IFKEKFAKAV GQGCVEIGSQ RYKLGVRLYY RVMESMLKSE EERLSIQNFS KLLNDNIFHM SLLACALEVV MATYSRSTSQ NLDSGTDLSF PWILNVLNLK AFDFYKVIES FIKAEGNLTR EMIKHLERCE HRIMESLAWL SDSPLFDLIK QSKDREGPTD HLESACPLNL PLQNNHTAAD MYLSPVRSPK KKGSTTRVNS TANAETQATS AFQTQKPLKS TSLSLFYKKV YRLAYLRLNT LCERLLSEHP ELEHIIWTLF QHTLQNEYEL MRDRHLDQIM MCSMYGICKV KNIDLKFKII VTAYKDLPHA VQETFKRVLI KEEEYDSIIV FYNSVFMQRL KTNILQYAST RPPTLSPIPH IPRSPYKFPS SPLRIPGGNI YISPLKSPYK ISEGLPTPTK MTPRSRILVS IGESFGTSEK FQKINQMVCN SDRVLKRSAE GSNPPKPLKK LRFDIEGSDE ADGSKHLPGE SKFQQKLAEM TSTRTRMQKQ KMNDSMDTSN KEEK
Molecular Weight	107.5 kDa.
Purity	>95% purity by SDS-PAGE
Endotoxin	<0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Recombinant Human pRB Protein is stored in 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA buffer.
Usage	For Research Use Only
Storage	Stored the protein at -70°C before use. Avoid repeated freeze thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.