Recombinant Human p53 Protein
Beta LifeScience
SKU/CAT #: BL-1406SG
Recombinant Human p53 Protein
Beta LifeScience
SKU/CAT #: BL-1406SG
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | GST |
Host Species | Human |
Accession | NM_000546 |
Synonym | TP53, LFS1, TRP53 |
Background | p53 is a key regulator of cell growth and acts as a tumor suppressor gene. Wild-type p53 gene can suppress transformation of rat embryo fibroblasts in cell culture by other oncogenes such as the adenovirus E1A and Ras (1). DNA tumor viruses such as SV40 large T antigen and the adenovirus E1A plus E1B-55Kd proteins bind to p53 and inactivate its tumor suppressor activities leading to cellular transformation. Mutational inactivation of the p53 gene is detected in more than 50% of human cancers. Mutation of p53 renders cancer cells more resistant to current cancer therapies due to lack of p53-mediated apoptosis (2). |
Description | Recombinant full-length human p53 was produced by baculovirus in Hi5 cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods. |
Source | Hi5 cells |
AA Sequence | Full Length |
Molecular Weight | ~79 kDa |
Purity | For specific purity information on a given lot, see related COA. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Formulation | Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
Stability | The recombinant protein is stable for up to 12 months at -70°C |
Usage | For Research Use Only |
Storage | Recombinant Human p53 Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |