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Recombinant Human CD2 Protein

Cat No.: BL-0012PS
CD2 Human Recombinant, sf9
Description Related products
tag His
host species Human
synonym T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, Erythrocyte receptor, Rosette receptor, CD2 antigen, CD2, T11, SRBC.
background CD2 which is also known as E-rosette receptor, T11 and lymphocytefunction antigen-2 (LFA-2) is expressed on T cells, thymocytes, and subset of natural killer cells. Human CD2 functions as the receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X). p56lck, p59fyn, CD3eta and CD3epsilon are tyrosine-phosphorylated after CD2 stimulation. CD2 plays a role in T cell activation, T- or NK-cell-mediated cytolysis, apoptosis in activated peripheral T cells, and regulation of T cell anergy.
description CD2 Human Recombinant expressed in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 194a.a. (25-209a.a.) and having a molecular weight of 22.3kDa (Molecular size on SDS-PAGE will appear at approximately 28-40 kDa).CD2 is expressed with a 6a.a. His tag at C-Terminus and purified by unique purification methods.
source Sf9
AA sequence ADPKEITNAL ETWGALGQDI NLDIPSFQMS DDIDDIKWEK TSDKKKIAQF RKEKETFKEK DTYKLFKNGT LKIKHLKTDD QDIYKVSIYD TKGKNVLEKI FDLKIQERVS KPKISWTCIN TTLTCEVMNG TDPELNLYQD GKHLKLSQRV ITHKWTTSLS AKFKCTAGNK VSKESSVEPVSCPEKGLDHH HHHH.
purity >95.0% as determined by SDS-PAGE.
endotoxin <1.0 EU per μg by the LAL method.
formulation CD2 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
stability Recombinant protein is stable for 12 months at -70℃
usage For Research Use Only
storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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