Recombinant Equine IL2 Protein
Beta LifeScience
SKU/CAT #: BL-0590PS
Recombinant Equine IL2 Protein
Beta LifeScience
SKU/CAT #: BL-0590PS
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | N/A |
Host Species | Equine |
Synonym | Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2. |
Background | IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. |
Description | Recombinant Equine Interleukin-2 expressed in E.Coli is a single, non-glycosylated, polypeptide chain containing 129a.a. (with a substitution of S for C - at position 141 compared with the wild type IL2) and having a molecular weight of 14.9kDa.The IL-2 Equine is purified by unique purification methods. |
Source | E.coli |
AA Sequence | APTSSSKRET QQQLKQLQMD LKLLLEGVNN NKNPKLSKML TFKINMPKKA TELKHLQCLE EELKPLEEML KNFLSKDIKE LMSNINVTVL GLKGSETRFT CEYDDETGTI VEFLNKWITF SQSIFSTMT. |
Purity | >97.0% as determined by SDS-PAGE and HPLC analyses. |
Endotoxin | <1.0 EU per μg by the LAL method. |
Bioactivity | The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 1.0 µg/ml, corresponding to a specific activity of > 1000 IU/mg. |
Formulation | Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 6.0. |
Stability | Recombinant protein is stable for 12 months at -70°C |
Usage | For Research Use Only |
Storage | Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |