Name: Recombinant Rhesus DKK1 Protein (256 Asn/Gln, His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1621
Availability: InStock

Recombinant Rhesus DKK1 Protein (256 Asn/Gln, His Tag)

Catalog No.: BLPSN-1621

Product Overview

Tag	His
Host Species	Rhesus
Accession	XP_001098844.1
Background	Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.
Description	A DNA sequence encoding the rhesus DKK1 (XP_001098844.1) precursor (Met 1-His 266) (256 Asn / Gln) was expressed, fused with a C-terminal His tag.
Source	HEK293
Predicted N Terminal	Thr 32
AA Sequence	Met 1-His 266
Molecular Weight	The recombinant rhesus DKK1 comprises 246 a.a. with a predicted molecular mass of 27.2 kDa. As a result of glycosylation, the apparent molecular mass of rhesus DKK1 is approximately 38-43 kDa in SDS-PAGE under reducing conditions.
Purity	>97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 0.5-3 ug/ml in the presence of 10 ng/mL of Wnt3a.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.