Recombinant Rat Neuroendocrine Convertase 2 (PCSK2) Protein (His)

Name: Recombinant Rat Neuroendocrine Convertase 2 (PCSK2) Protein (His)
Brand: Beta LifeScience
SKU: BLC-09244P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Rat Neuroendocrine Convertase 2 (PCSK2) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P28841
Target Symbol	PCSK2
Synonyms	Pcsk2; Nec-2; Nec2; Rpc2; Neuroendocrine convertase 2; NEC 2; EC 3.4.21.94; KEX2-like endoprotease 2; Prohormone convertase 2; Proprotein convertase 2; PC2
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	GYRDINEIDINMNDPLFTKQWYLFNTGQADGTPGLDLNVAEAWELGYTGKGVTIGIMDDGIDYLHPDLAYNYNSDASYDFSSNDPYPYPRYTDDWFNSHGTRCAGEVSAAASNNICGVGVAYNSKVAGIRMLDQPFMTDIIEASSISHMPQLIDIYSASWGPTDNGKTVDGPRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYDDCNCDGYASSMWTISINSAINDGRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANVDLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQNPEKIPPTGKLVLTLQTNACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGLLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKQELEEELDEAVERSLQSILRKN
Expression Range	109-637aa
Protein Length	Full Length of Mature Protein
Mol. Weight	62.3kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells.
Subcellular Location	Cytoplasmic vesicle, secretory vesicle. Secreted.
Protein Families	Peptidase S8 family, Furin subfamily
Database References	KEGG: rno:25121 STRING: 10116.ENSRNOP00000007249 UniGene: Rn.89052

Gene Functions References

The nutrient sensor sirtuin 1 regulates the production of CRH post-translationally by affecting PC2 post-translationally by affecting PC2. PMID: 26755731
The presence of PC and GOAT in the cells, as well as n-octanoic acid in the culture medium, was necessary to produce n-octanoyl ghrelin. PMID: 19628676
The prohormone convertase (PC) enzyme PC1 together with PC2 and PC5 may represent a redundant system to ensure production of biologically active cholecystokinin in rat brain PMID: 14608596
Leptin regulates hypothalamic Pcsk2 expression and requires prohormone processing into mature peptides. PMID: 15286802
study demonstrates abundant expression of PC2 mRNA in rat brain and shows that thyroid status regulated PC2 expression in the hypothalamus and cortex [PC2] PMID: 15585599
Expression levels of POMC processing enzymes prohormone convertases 1 and 2 (PC1/PC2) were not significantly affected by high dietary salt intake in either WKY or SP-SHR tissues. PMID: 17531155
our data anticipate the possibility that carboxypeptidase-E, prohormone convertase 1, and prohormone convertase 2 might be potential target for the maturation of somatostatin PMID: 17543468
PC1/PC3 and PC2 (specific to neural and endocrine cells) are absent in undifferentiated bone marrow stromal stem cells, their expression is initiated upon the induction of differentiation PMID: 17556096
brain ischemia attenuates PC2 and PC2-mediated neuropeptide processing. PMID: 19142196
PC2, CPE and preproNPY protein expression levels were compared among mature retina tissue, RGC-5 cells and its differentiated cells; differentiated cells or tissues had higher or highest PC2 activity PMID: 19428990

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.