Recombinant Rat Glutamate Receptor Ionotropic, Nmda 1 (GRIN1) Protein (His)

Name: Recombinant Rat Glutamate Receptor Ionotropic, Nmda 1 (GRIN1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-00742P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Rat Glutamate Receptor Ionotropic, Nmda 1 (GRIN1) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P35439
Target Symbol	GRIN1
Synonyms	(GluN1)(Glutamate [NMDA] receptor subunit zeta-1)(N-methyl-D-aspartate receptor subunit NR1)(NMD-R1)
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	RAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYNWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEARELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGIIGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTMSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCIDLLIKLARTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQ
Expression Range	19-559aa
Protein Length	Partial
Mol. Weight	64.7 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition.
Subcellular Location	Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. Cell junction, synapse, postsynaptic density.
Protein Families	Glutamate-gated ion channel (TC 1.A.10.1) family, NR1/GRIN1 subfamily
Database References	KEGG: rno:24408 STRING: 10116.ENSRNOP00000029227 UniGene: PMID: 29779156 The relative gene expression of rostral ventral lateral medulla (RVLM) NMDA and AMPA glutamate-gated receptor subunits and protein concentration of select receptor subunits did not differ between young and aged rats, and there were no age-related differences in the expression of RVLM ionotropic GABAA and Gly receptors, or of protein concentration of select GABAA subunits. PMID: 28263869 NMDAR1 is significantly activated in hypothalamic paraventricular nucleus in rats with heart failure. PMID: 28958939 Study demonstrates that in the rat model, endometriosis can be associated with a decrease in mu opioid receptors (MOR)immunoreactivity within neuronal compartments in addition to a shift in MOR and N-methyl-d-aspartate subunit receptor (NR1) receptor expression within the ventral periaqueductal gray. PMID: 27089914 restoring chloride homeostasis normalizes both presynaptic and postsynaptic NMDAR activity increased by nerve injury in the spinal dorsal horn. PMID: 27160909 tPA is a ligand of the N-terminal domain of the obligatory GluN1 subunit of NMDAR acting as a modulator of their dynamic distribution at the neuronal surface and subsequent signaling. PMID: 27831563 Allosteric interactions occur between the glutamate-binding GluN2 subunits in triheteromeric GluN1/2A/2B NMDARs. This allosterism is dominated by the GluN2A subunit and results in functional properties not predicted by those of diheteromeric GluN1/2A and GluN1/2B NMDARs. PMID: 28384476 Single unilateral infusion of NMDA at mild dose into the hippocampal formation induced chronic, focal neurodegeneration accompanied by severe and progressive neuroinflammation and affected preferentially principal neurons; GluN1 and GluN2B subunits of NMDA receptor were significantly upregulated up to 3 days after the NMDA infusion, while GABAA alpha5 subunit was downregulated at 30 days after the lesion PMID: 26930443 In neuronal cell line (NG108-15) in vitro, hypoxia caused a significant increase in mRNA and protein for NMDA-NR1 mRNA and protein levels. PMID: 27810863 Interferon gamma (IFNgamma) enhance the amplitude of NMDA-induced inward currents in substantia gelatinosa neurons via microglial IFNgamma receptors and CCL2/CCR2 signaling. PMID: 27094552 shRNA-mediated knockdown of Shank3 results in up-regulation of the NMDA receptor subunit GluN1 in axon terminals. PMID: 26725465 young adult runners demonstrated a significant increase in total GluN (1 and 2A) subunit expression in the dorsal hippocampus, and an opposing effect in the ventral hippocampus. PMID: 26220171 NMDA receptor hyperfunction in lipopolysaccharide-treated kidneys was demonstrated by NR1 upregulation, particularly in renal tubules. IL-1beta-mediated NMDA receptor hyperfunction participates in lipopolysaccharide-induced tubular damage. PMID: 26133372 This study demonstrated that the Male prenatal chronic mild stress rats expressed significantly lower NR1 levels in the prefrontal cortex. and lower in femal rat hippocampus. PMID: 26071959 Hepatic encephalopathy affects the gene expression of Grin1 in brain with a site-specific pattern. PMID: 25896221 Remifentanil induces an increase in GluN1 mRNA and protein levels in primary rat dorsal horn neurons, which may partially account for remifentanil-induced hyperalgesia. PMID: 25867330 Contacts specific to GluN1 and GluN2A NMDA receptors, which brace the heterodimer interface within the ligand binding domain, stabilize actively gating receptor conformations and result in longer bursts and slower deactivations. PMID: 26370091 MARK/Par1 is activated downstream of NMDA receptors in primary hippocampal neurons. PMID: 25932647 Dorsal hippocampal receptor complex levels containing NR1/GluA1/GluA2 were modulated by long term potentiation induction. PMID: 24442866 Long-term potentiation (LTP) and long-term depression (LTD) signaling converge towards phosphatidylinositol-(3,4,5)-trisphosphate (PIP3) upregulation, but PTEN acts as an LTD-selective switch that determines the outcome of PIP3 accumulation. PMID: 25335889 we found that NMDARs are released from the ER only when two asparagine residues in the GluN1 subunit (Asn-203 and Asn-368) are N-glycosylated PMID: 26045554 Long-term blockade of the NMDA receptor in developing rat hippocampal neurons significantly increased NR1 subunit expression, and that this was associated with an alteration in neuronal activity. PMID: 25937004 KN-62, a selective inhibitor of rat brain Ca(2+)/calmodulin-dependent protein kinase II (CaMKII), diminished the increase in serine phosphorylation of GluR6. PMID: 16126302 GluN1 amino-terminal domain adopts a more open conformation when coassembled with GluN2A than with GluN2B. PMID: 25829490 Data show that the receptor tyrosine kinase-like orphan receptor 2 (RoR2) functions as a Wnt5a receptor required to maintain basal NMDA receptors (NMDARs)-mediated synaptic transmission. PMID: 25825749 In summary, we identified genes that are downregulated in the PFC of SHR, and might be related to the behavioral abnormalities exhibited by this strain. PMID: 25041985 NMDAR1 were increased in the prefrontal cortex (PC), hippocampus and amygdala of CHF, and decreased in the hippocampus, amygdala and NAc of CHF stressed. PMID: 25772108 The ligand binding domains (LBD) of the GluN1 and GluN2A subunits of the NMDAR heterodimerize only when both coagonists, Glu and Gly/d-Ser, bind to their respective sites on GluN2 and GluN1. PMID: 25544544 acute 5-HT7 receptor activation increases NR1 NMDA receptor subunit phosphorylation and differentially alters the phosphorylation state of the NR2B and NR2A subunits. PMID: 23672716 The hippocampus of fetuses gestated under light:dark photoperiod (12:12 LD) displayed daily oscillatory expression of the clock genes Bmal1 and Per2, clock-controlled genes Mtnr1b, Slc2a4, Nr3c1 and NMDA receptor subunits 1B-3A-3B PMID: 24663672 Redox regulation of NMDAR synaptic responses are reglated by antoxidant enzymes,SOD1. PMID: 24388786 Antibody-mediated downregulation of surface NMDARs engages homeostatic synaptic plasticity mechanisms, which may inadvertently contribute to immune-mediated encephalitis progression. PMID: 24916964 NR1 protein expression in the rat retina by immunofluorescence double labeling: the NR1 subunit is expressed more widely than was previously appreciated PMID: 22512920 data support the argument that PS to pregnant dams could induce depression-like behavior, which may be involved with abnormal expression of NR1 and NR2A in specific brain regions, and MK-801 may have antidepressant-like effects on the juvenile offspring PMID: 24278457 The results indicate that NMDAR1 and CREB are involved in the ocular dominance forming process and play a role in vision plasticity PMID: 23381906 alpha7nAchR/NMDAR coupling may selectively affect some aspects of learning and memory. PMID: 24360204 A single amino acid in GluN1 that is critical for priming of NMDA receptors by glycine, was identified. PMID: 23941530 The altered NR1 could have potential impact on anxiety-like behavior in the adult offspring exposed to Prenatal stress. PMID: 24029697 this study shows the crystal structure of the intact heterotetrameric GluN1-GluN2B NMDA receptor ion channel at 4 angstroms. PMID: 24876489 Crystallization and structural analysis of the GluN1 and GluN3A ligand binding domains of the rat NMDA receptor subunits Grin1 and Grin3a. PMID: 23972471 We developed a method to provide selective cell-surface expression of recombinant GluN1/GluN2A/GluN2B triheteromers and compared properties of these receptors with those of GluN1/GluN2A and GluN1/GluN2B diheteromers. PMID: 24607230 Virtually all bulbospinal neurons in the rostral ventrolateral medulla express NR1. PMID: 23562375 Exercise training decreases persistent postsurgical pain via decreased expression of spinal cord NR1, TNF-alpha, and IL-6. PMID: 23640243 Crystal structure and pharmacological characterization of a novel N-methyl-D-aspartate (NMDA) receptor antagonist at the GluN1 glycine binding site. PMID: 24072709 Data from molecular modeling suggest that GluN1-containing NMDA receptors can enter a conformation or mode that allows the pore to gate with high probability of burst of activity. PMID: 23578394 NR1 mRNA expression increased in the hippocampus in a model of chronic temporal lobe epilepsy. PMID: 23392471 Data indicate that AMPA receptors have an alternating subunit arrangement of GluA1/GluA2, whereas NMDA receptors adopt an adjacent arrangement of GluN1/GluN2A, whereas NMDA receptors adopt an adjacent arrangement. PMID: 23760273 Feeding-specific circuits between the nucleus accumbens shell and the lateral hypothalamus are regulated by NMDA receptors (and GABA(A) receptors). PMID: 23164617 Findings suggest a mechanism in which NMDAR-dependent phosphorylation of ERK1/2 modulates satiation signals via synapsin I phosphorylation in vagal afferent endings. PMID: 23715865 GluN1, an NMDA receptor subunit in the adult rat hippocampus undergoes similar changes after 5 minutes in an open field and after long term potentiation-induction. PMID: 23383317

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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