Recombinant Rat Ephrin-B1 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1893
Recombinant Rat Ephrin-B1 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1893
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | Fc |
| Host Species | Rat |
| Accession | P52796 |
| Background | Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays. |
| Description | A DNA sequence encoding the rat EFNB1 (P52796) (Met1-Thr229) was expressed, fused with the Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Ala 25 |
| AA Sequence | Met1-Thr229 |
| Molecular Weight | The recombinant rat EFNB1 comprises 446 a.a. and predicts a molecular mass of 49.4 kDa. The apparent molecular mass of the rat EFNB1 is approximately 58 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Purity | >98% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Immobilized mouse EphB3-His at 10 ug/ml (100 ul/well) can bind ratEFNB1-Fc, The EC50 of ratEFNB1-Fc is 6.1-14.2 ng/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
