Name: Recombinant Rat Ephrin-A1 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1888
Availability: InStock

Recombinant Rat Ephrin-A1 Protein (His Tag)

Catalog No.: BLPSN-1888

Product Overview

Tag	His
Host Species	Rat
Accession	P97553
Background	EPH-related receptor tyrosine kinase ligand 1 (abbreviated as Ephrin-A1) also known as ligand of eph-related kinase 1 or EFNA1, is a member of the ephrin (EPH) family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A1/EFNA1 and its Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A1 and one of its receptor EphA2 were expressed in xenograft endothelial cells and also tumor cells and play a role in human cancers, at least in part by influencing tumor neovascularization.
Description	A DNA sequence encoding the rat EFNA1 (P97553) (Met1-His181) was expressed, fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Ala 18
AA Sequence	Met1-His181
Molecular Weight	The recombinant rat EFNA1 comprises 175 a.a. and predicts a molecular mass of 20.7 kDa. The apparent molecular mass of the rat EFNA1 is approximately 27 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its binding ability in a functional ELISA.Immobilized Rat EFNA1-His can bind biotinylated mouse EPHA2-His with a linear range of 6.25-200 ng/ml.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.