Name: Recombinant Rat Cripto Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1432
Availability: InStock

Recombinant Rat Cripto Protein (His Tag)

Catalog No.: BLPSN-1432

Product Overview

Tag	His
Host Species	Rat
Accession	XP_001056317.2
Background	Cripto/TDGF1 is a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family. EGF-CFC family member proteins share a variant EGF-like motif, a conserved cysteine-rich domain, and a C-terminal hydrophobic region. Before gastrulation, Cripto is asymmetrically expressed in a proximal-distal gradient in the epiblast, and subsequently is expressed in the primitive streak and newly formed embryonic mesoderm. These proteins play key roles in intercellular signaling pathways during vertebrate embryogenesis. Mutations in Cripto/TDGF1 can cause autosomal visceral heterotaxy. Cripto/TDGF1 is involved in left-right asymmetric morphogenesis during organ development. Cripto signalling is essential for the conversion of a proximal-distal asymmetry into an orthogonal anterior-posterior axis. The mechanism of inhibitory effects of the Cripto includes both cancer cell apoptosis, activation of c-Jun-NH(2)-terminal kinase and p38 kinase signaling pathways and blocking of Akt phosphorylation. Thus, Cripto is a unique target, and Immunohistochemistry to Cripto could be of therapeutic value for human cancers.
Description	A DNA sequence encoding the rat TDGF1 (XP_001056317.2) (Met1-Cys143) was expressed with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Phe 17
AA Sequence	Met1-Cys143
Molecular Weight	The recombinant rat TDGF1 comprises 138 a.a. and predicts a molecular mass of 15.5 kDa. The apparent molecular mass of the recombinant protein is approximately 22 and 19 kDa in SDS-PAGE under reducing conditions.
Purity	(32.6+48.9)% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to bind with rat ACVR1B-Fc in a functional ELISA.
Formulation	Lyophilized from sterile PBS, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.