Recombinant Rat CNTF Protein
Beta LifeScience
SKU/CAT #: BLPSN-1360
Recombinant Rat CNTF Protein
Beta LifeScience
SKU/CAT #: BLPSN-1360
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | N/A |
Host Species | Rat |
Accession | P20294 |
Background | Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that have functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It's actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi- subunit receptor complex, consisting of an extracelluar CNTF binding subunit (CNTFalpha) and two transmembrane signal transduction proteins: glycoprotein gp13 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocyteands may be relevant in reducing tissue destruction during inflammatory attacks. CNTF also is a survival factor for neurons of the peripheral sensory sympathetic and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors. |
Description | A DNA sequence encoding the rat CNTF (P20294) (Met 1-Met 200) was expressed and purified. |
Source | E.coli |
Predicted N Terminal | Met 1 |
AA Sequence | Met 1-Met 200 |
Molecular Weight | The recombinant rat CNTF consisting of 200 a.a. and has a calculated molecular mass of 22.9 kDa. It migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions. |
Purity | >92% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | 1. Measured by its binding ability in a functional ELISA. Immobilized rat CNTF at 10 ug/ml (100 ul/well) can bind biotinylated rat CNTFR-His (aa 1-346) with a linear range of 1.56-50.0 ng/ml.2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 10-40 ng/ml. |
Formulation | Lyophilized from sterile 50mM Tris, 0.1M NaCl, pH 7.5. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |