Name: Recombinant Rat Cadherin 8 Protein
Brand: Beta LifeScience
SKU: BLPSN-0503
Availability: InStock

Catalog No.: BLPSN-0503

Product Overview

Tag	N/A
Host Species	Rat
Accession	NP_445845.2
Background	Cadherins are integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Type I cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of five subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Cadherin 8, also known as CDH 8, is a type II classical cadherin belonging to the cadherin superfamily. As mainly expressed in brain, CDH8 is found in certain nerve cell lines, such as retinoblasts, glioma cells and neuroblasts, and is putatively involved in synaptic adhesion, axon outgrowth and guidance. Human Cadherin 8 is a 799 amino acid single-pass type Itransmembrane protein with a putative 29 aa signal sequence, and a 32 aa propeptide, a 56 aa mature extracellular domain, a 21 aa transmembrane domain and a 157 aa cytoplasmic domain. The human, mouse and rat proteins share approximately 98% homology.
Description	A DNA sequence encoding the rat CDH8 (NP_445845.2)(Met1-Met621) was expressed with six amino acids (LEVLFQ) at the C-terminus.
Source	HEK293
Predicted N Terminal	Ala 30
AA Sequence	Met1-Met621
Molecular Weight	The recombinant rat CDH8 comprises 599 a.a. and predicts a molecular mass of 65.9 kDa. The apparent molecular mass of the recombinant protein is approximately 76-85 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>80% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.