Recombinant Rat BCAM Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0364
Recombinant Rat BCAM Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0364
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Rat |
Accession | Q9ESS6 |
Background | The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes. |
Description | A DNA sequence encoding the rat BCAM (Q9ESS6) (Met1-Ala543) was expressed, fused with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Glu 26 |
AA Sequence | Met1-Ala543 |
Molecular Weight | The recombinant rat BCAM comprises 529 a.a. and predicts a molecular mass of 58.2 kDa. The apparent molecular mass of the recombinant protein is approximately 68 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Purity | >90% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells.When cells are added to coated plates(10 ug/mL,100uL/well), approximately 20-50% will adhere after 1 hour at 37°C. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |