Recombinant Rat Angiopoietin-1 (ANGPT1) Protein (His)

Name: Recombinant Rat Angiopoietin-1 (ANGPT1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-07404P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Rat Angiopoietin-1 (ANGPT1) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	O35460
Target Symbol	ANGPT1
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	C-6His
Target Protein Sequence	SNQRRSPENGGRRYNRIQHGQCAYTFILPEHDGNCRESATEQYNTNALQRDAPHVETDFSSQKLQHLEHVMENYTQWLQKLENYIVENMKSEMAQIQQNAVQNHTATMLEIGTSLLSQTAEQTRKLTDVETQVLNQTSRLEIQLLENSLSTYELEKQLLQQTNEILKIQEKNSLLEHKILEMEGKHKEELDTLKEEKENLQGLVTRQTFIIQELEKQLSRATSNNSVLQKQQLELMDTVHNLVSLCTKEVLLKGGKREEEKPFRDCADVYQAGFNKSGIYTIYFNNMPEPKKVFCNMDVNEGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNQKQNYRLYLKGHTGTAGKQSSLILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTMMIRPLDF
Expression Range	20-497aa
Protein Length	Full Length of Mature Protein
Mol. Weight	56.3 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
Subcellular Location	Secreted.
Database References	KEGG: rno:89807 STRING: 10116.ENSRNOP00000007979 UniGene: Rn.161953

Gene Functions References

Overexpression of Ang-1 enhanced the tube formation, migration, and proliferation ability of endothelial progenitor cells. EPC transplantation facilitated occlusion of carotid aneurysms. PMID: 29197611
The study showed for the first time that ANG-1 plays a vital role in the early rather than late phase of adjuvant-induced arthritis pathogenesis making it an attractive non-invasive tool for early diagnosis and prediction of disease prognosis PMID: 24664592
Ang-1-EPC improved cell survival during inflammation. PMID: 24606182
Ang1 was implicated in ASC-induced suppression of neointimal formation. PMID: 23486192
Endothelin-1 regulates ANG-1 production and vascular endothelial growth factor (VEGF)A in proliferating astrocytes. PMID: 22927341
responsible for the vascular hyperreactivity at early hemorrhagic shock through the Tie2-Akt-eNOS pathway PMID: 22869617
The expression of Ang-1 mRNA and TSP-1 in renal tissue of rats after subtotal nephrectomy was significantly up-regulated. PMID: 19821086
Angiopoietin-1 preconditioning enhances survival and functional recovery of mesenchymal stem cell transplantation PMID: 22843181
Prolonged exposure of high concentration of oxygen may cause impairment of lung vascular development by inhibiting expression of Ang-1 in neonatal rats. PMID: 22336306
The change of Ang-1, which is early upregulation and late downregulation, exists in diabetic renal tissue. PMID: 17294737
Wenyang Huoxue Recipe can up-regulate the expression of Ang-l mRNA rats with chronic aristolochic acid nephropathy. PMID: 18471417
Expression of angiopoietin-1 and the angiopoietin receptor dropped markedly after stroke in SHR stroke-prone rats, but was not different after ischemia in SHR and WKY rat strains PMID: 11822892
ang 1 stabilizes testicular microvessels under basal conditions and that a shift in this balance caused by increased ang 2, together with increased VEGF-A, allows vascular leakage, high endothelial cell proliferation PMID: 12773423
Rats engineered to express angiopoietin 1 (Ang-1) constitutively in the lung develop severe pulmonary hypertension. PMID: 14512515
Overexpression of angiopoietin-1 using an adeno-associated virus vector causes pulmonary hypertension in rats. These data provide a novel physiologic animal model for pulmonary hypertension. PMID: 14759414
E2 increases Ang-1 and enhances capillary density in brain under basal conditions through estrogen receptor-alpha, priming the middle cerebral artery territory for survival after experimental focal ischemia PMID: 15637314
Ang-1 promotes neurite outgrowth from dorsal root ganglia cells positive for Tie2. PMID: 15714275
It is concluded that adipocyte-secreted Ang-1 supports neurite outgrowth, which is involved in synaptogenesis. The novel function of Ang-1 appears to play a physiological role in WAT plasticity. PMID: 16493688
Ang1 protects cardiomyocytes from oxidative stress-induced apoptosis by regulating the activity of Proto-Oncogene Proteins c-akt PMID: 17559808
ANGPT1 is necessary for follicular development induced by gonadotropins. PMID: 17989359
Expression of angiopoietin-1 significantly decreased in the pulmonary arteries in chronic hypoxic pulmonary hypertension. PMID: 18073453
Abnormal expression and reciprocal egulation of Angpt1 may play important roles in the development of chronic allograft nephropathy in rat renal allografts. PMID: 18929864
Ang1 modifies basal microvessel permeability coefficients, in keeping with previous reports demonstrating reduced solute flux in inflamed vessels. PMID: 19297368
Ang1 induces neurite outgrowth in PC12 cells in a Tie2-independent, beta1-integrin-FAK-PI3K-Akt-dependent manner. NGF and Ang1 mediate neurite outgrowth via two independent signaling mechanisms. PMID: 19379779
Vascular endothelial growth factor and angiopoietin-1 are involved in liver regeneration following partial hepatectomy. PMID: 11549276

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.