Name: Recombinant Rat Aminopeptidase A Protein (aa 41-945, His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0179
Availability: InStock

Recombinant Rat Aminopeptidase A Protein (aa 41-945, His Tag)

Catalog No.: BLPSN-0179

Product Overview

Tag	His
Host Species	Rat
Accession	P50123
Synonym	ENPEP
Background	ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.
Description	A DNA sequence encoding the mature form of rat ENPEP (P50123-1) (Arg41-Pro945) was expressed,with a His tag at the N-terminus.
Source	Baculovirus-Insect Cells
Predicted N Terminal	His
AA Sequence	Arg41-Pro945
Molecular Weight	The recombinant rat ENPEP consists of 921 a.a. and predicts a molecular mass of 106 KDa. It migrates as an approximately 116 KDa band in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin (Glu-AMC).The specific activity is >1, 500 pmoles/min/ug.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.