Recombinant Neisseria Meningitidis Serogroup B Quinolinate Synthase (NADA) Protein (His)

Name: Recombinant Neisseria Meningitidis Serogroup B Quinolinate Synthase (NADA) Protein (His)
Brand: Beta LifeScience
SKU: BLC-02416P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Neisseria meningitidis serogroup B (strain MC58) nadA.

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Product Overview

Description	Recombinant Neisseria Meningitidis Serogroup B Quinolinate Synthase (NADA) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q9K105
Target Symbol	NADA
Synonyms	nadA; NMB0394; Quinolinate synthase A; EC 2.5.1.72
Species	Neisseria meningitidis serogroup B (strain MC58)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRLKDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAGVRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAAVKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYICRETGADMLLWQGSCIVHNEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVATDLGILHEMQKQAPDKQFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEILLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA
Expression Range	1-370aa
Protein Length	Full Length
Mol. Weight	44.2 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.
Subcellular Location	Cytoplasm.
Protein Families	Quinolinate synthase A family, Type 1 subfamily
Database References	KEGG: nme:NMB0394 STRING: 122586.NMB0394

Gene Functions References

Data show that a protective conformational epitope is located in the head of Neisserial adhesin A (NadA). PMID: 25404323
The prevalence and genetic diversity of pilE and nadA were investigated in the prevalent serogroups and clonal complexes of N. meningitidis isolated in China. PMID: 23290624
The authors demonstrate by reporter gene fusion assays that alterations of the FarR binding site within the nadA promoter are sufficient to induce transcription of nadA. PMID: 21292554
Our results suggest that NadA expression is under both stochastic and tight environmental-sensing regulatory control PMID: 20041170
NmFarR is a negative transcriptional regulator of the adhesin/invasin NadA. PMID: 19400792

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.