Recombinant Mouse VCAM1 Protein (His & Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-4763
Recombinant Mouse VCAM1 Protein (His & Fc Tag)

Recombinant Mouse VCAM1 Protein (His & Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-4763
Catalog No.: BLPSN-4763

Product Overview

Tag His&Fc
Host Species Mouse
Accession NP_035823.3
Synonym CD106, Vcam-1
Background Vascular cell adhesion molecule 1 (VCAM-1), also known as CD16, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and alpha4β7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description A DNA sequence encoding the extracellular domain (Met 1-Glu 698) of mouse VCAM1 (NP_035823.3) precursor was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.
Source HEK293
Predicted N Terminal Phe 25
AA Sequence Met 1-Glu 698
Molecular Weight The recombinant mouse VCAM1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 922 a.a. and has a predicted molecular mass of 102 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmVCAM1/Fc monomer is approximately 110-120 kDa due to glycosylation.
Purity >90% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When cells are added to VCAM1-coated plates (10 ug/ml, 100 ug/well), approximately >70% cells will adhere specifically.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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