Recombinant Mouse Uteroglobin Protein (His Tag)

Name: Recombinant Mouse Uteroglobin Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-4756
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Mouse
Accession	Q06318
Synonym	CC10, CC16, CCSP, PCB-BP, UG, UGB, Utg
Background	Uteroglobin (UG), also known as Secretoglobin 1A member 1 (SCGB1A1), Blastokinin, Clara cell secretor protein (CCSP) or Clara cell-specific 1-kDa protein (CC1), is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is mainly expressed in lung, with anti-inflammatory/immunomodulatory properties. Previous in vitro studies demonstrated that CCAAT/enhancer-binding proteins (C/EBPs) are the major transcription factors for the regulation of SCGB1A1 gene expression, whereas FOXA1 had a minimum effect on the transcription. Uteroglobin is a multifunctional protein with antiinflammatory/immunomodulatory properties. Uteroglobin inhibits soluble phospholipase A(2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, Uteroglobin manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities. The tissue-specific expression of the Uteroglobin gene is regulated by several steroid hormones, although a nonsteroid hormone, prolactin, further augments its expression in the uterus. Based on its anti-inflammatory and antiallergic properties, Uteroglobin is a potential drug target. The mechanism of Uteroglobin action is likely to be even more complex as it also functions via a putative receptor-mediated pathway.
Description	A DNA sequence encoding the mouse SCGB1A1 (Q06318) (Met 1-Phe 96) was expressed, with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Asp 22
AA Sequence	Met 1-Phe 96
Molecular Weight	The recombinant mouse SCGB1A1 consists of 86 a.a. and has a predicted molecular mass of 9.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm SCGB1A1 is approximately 12 kDa.
Purity	>88% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cell line. When 5 x 10E4 cells/well are added to SCGB1A1 coated plates (5 ug/ml with 100 ul/well), approximately >40% will adhere after 1 hour at 37°C.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
Subcellular Location	Secreted.
Protein Families	Secretoglobin family
Database References	KEGG: mmu:22287 STRING: 10090.ENSMUSP00000025554 UniGene: Mm.2258
Tissue Specificity	Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).

Gene Functions References

the present results demonstrate that rCC16 has therapeutic effects on COPD by downregulating proinflammatory factors via the NFkappaB pathway. PMID: 29956762
This study extends the function of the Scgb1a1-expressing epithelial cell population as a major mediator of the antiviral response. It PMID: 29593031
Chronic P. aeruginosa inflammation resulted in chronic bronchitis and emphysematous changes in CCSP-deficient mice. PMID: 27703342
CC16 protects lungs from cigarette smoke (CS)-induced injury by reducing lung NF-kappaB activation. CS-induced airway CC16 deficiency increases CS-induced pulmonary inflammation and injury and likely contributes to the pathogenesis of COPD. PMID: 25700379
Depletion of bone marrow CCSP-expressing cells delays airway regeneration. PMID: 25409745
Serum CC-16 is associated with disease progression in chronic obstructive pulmonary disease (COPD). However, the absence of CC-16 does not appear to modify the risk of cigarette-related COPD in mice. PMID: 24245748
These findings indicate that CCSP has a regulatory role in obliterative bronchiolitis. PMID: 23997179
the role of clara cells and clara cell secretory protein in lung radiation injury exacerbated by viral infection PMID: 23621375
AAV2/9-CC10 vector virus guaranteed sufficient CC10 expression and had an anti-inflammatory effect in asthmatic mice. PMID: 23013277
Claudin-3 and Clara cell 10 kDa protein were identified as possible markers of early tobacco smoke-induced epithelial injury along alveolar ducts. PMID: 22659244
Scgb1a1-expressing cells, most likely Clara cells, are a major cell type that gives rise to alveolar type I and II cells during the regeneration of alveolar epithelia in response to severe pulmonary damage in mice. PMID: 23119022
These results indicate that CC10 gene transfer may inhibit airway inflammation through suppressing the activation of NF-kappaB. PMID: 22558282
Clara cell secretory protein expression is elevtaed in lungs after long-term n-hexane inhalation exposure. PMID: 20853679
CCSP promoter has a role in alveolar development PMID: 20693404
The expression of CC10 is downregulated in sinonasal mucosa in bacterial chronic rhinosinusitis. PMID: 19119605
Expression of SCGB1A1 mRNA was not decreased in vivo in the absence of C/EBPs. PMID: 21224212
The differential expression of mRNA by both airway level and lung region was determined for Clara cell secretory protein. PMID: 20852037
Lack of uteroglobin in mice enhances colonization of B16F10 melanoma cells in the lungs. PMID: 20118237
macrophages from Clara cell-depleted and CCSP(-/-) mice displayed increased Toll-like receptor 4 surface expression PMID: 19423773
Uteroglobin promoter-targeted c-MYC expression in transgenic mice cause hyperplasia of Clara cells and malignant transformation of T-lymphoblasts and tubular epithelial cells PMID: 11817538
Data show that Clara cell secretory protein (CCSP) is required for the appearance of secretory granules and that functional changes to Clara cells that result from CCSP deficiency lead to alterations in the composition of epithelial lining fluid. PMID: 12151308
demonstrate synergistic transactivation by C/EBPalpha and Nkx2.1 in the regulation of the CCSP gene PMID: 12161423
An immunohistochemical analysis was made of the expression of this protein in a transgenic model of mouse lung carcinogenesis. PMID: 12699910
Restoration of CCSP in the airways of CCSP-deficient mice abrogates the increase in viral persistence, lung inflammation, and airway hyperreactivity induced by respiratory syncytial virus infection. PMID: 12847279
Multiple mechanisms for repression of the Clara cell secretory protein gene during hyperoxia. PMID: 14500549
Data show that 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone exposure of Clara cell 10-kDa protein knock-out mice causes a significantly higher incidence of airway epithelial hyperplasia and lung adenomas compared with wild type littermates. PMID: 15148323
plays a direct role in the regulation of T-cell-mediated inflammatory responses. PMID: 15356574
We propose that mechanical activation of the cPLA2 pathway contributes to acute high PIP-induced lung injury and that CCSP may reduce this injury through inhibition of the cPLA2 pathway and reduction of proinflammatory products produced by this pathway. PMID: 15608088
Uteroglobin suppresses SCCA gene expression associated with airway inflammation in asthma PMID: 15677460
uteroglobin plays important roles in maintaining homeostasis in organs that are vulnerable to inadvertent stimulation of FP-mediated inflammatory response by inhibiting the prostaglandin F2alpha receptor PMID: 16061484
A critical role for uteroglobin in preventing the development of pulmonary fibrosis. PMID: 16872605
These results suggest that both long- and short-range paracrine signaling between nonciliated secretory cells and cells of the immune system and epithelium impact modification of cell type-specific proteins. PMID: 17384087
Treatment of lung neoplasms with bexarotene resulted in decreased mRNA expression. PMID: 17849452
Clara cells accumulated Clara cell secretory protein (CCSP) in Munc13-2-deficient mice. PMID: 18258655
preferentially expressed in mouse model of allergen-induced airway inflammation and remodeling PMID: 19322781

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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