Recombinant Mouse UCHL3 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-4725
Recombinant Mouse UCHL3 Protein (His Tag)

Recombinant Mouse UCHL3 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-4725
Catalog No.: BLPSN-4725

Product Overview

Tag His
Host Species Mouse
Accession Q9JKB1
Background Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to thepeptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.
Description A DNA sequence encoding the mouse UCHL3 (Q9JKB1) (Glu 2-Ala 230) was expressed, with a His tag at the N-terminus.
Source E.coli
Predicted N Terminal Met
AA Sequence Glu 2-Ala 230
Molecular Weight The recombinant mouse UCHL3 consisting of 240 a.a. and has a calculated molecular mass of 27.5 kDa. rm UCHL1 migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.
Purity >97% as determined by SDS-PAGE
Endotoxin Please contact us for more information.
Bioactivity Measured by the hydrolysis of UbiquitinAMC. The specific activity is >14000 pmoles/min/ug.
Formulation Lyophilized from sterile 50mM Tris, 150mM NaCl, 20% glycerol, pH 7.7.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recently viewed