Recombinant Mouse TIM-1 Protein (C-6His)

Name: Recombinant Mouse TIM-1 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-1186NP
Availability: InStock

BL-1186NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Hepatitis A Virus Cellular Receptor 1 Homolog is produced by our Mammalian expression system and the target gene encoding Tyr22Thr212 is expressed with a 6His tag at the C-terminus.
Accession	Q5QNS5
Synonym	Hepatitis A virus cellular receptor 1 homolog; HAVcr-1; Kidney injury molecule 1; KIM-1; T cell membrane protein 1; TIM-1
Gene Background	TIM-1/KIM-1/HAVCR belongs to the immunoglobulin superfamily that cosisits 305 amino acid (aa). It is expressed by stimulated T-cells. TIM-1/KIM-1/HAVCR may play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. And may have a role in kidney injury and repair. Belongs to the T-cell and airway phenotype regulator (Tapr) locus, a single chromosomal region that confers reduced T-helper type 2 responsiveness and protects against airway hyperactivity (AHR), the hallmark of human asthma.
Molecular Mass	21.8 KDa
Apmol Mass	35-50 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	Immunoglobulin superfamily, TIM family
Database References	KEGG: mmu:171283 STRING: 10090.ENSMUSP00000043827 UniGene: PMID: 28874468 findings provide evidence for the role of Tim-1 in the induction of a cytokine storm phenomenon and the pathogenesis of Ebola virus disease PMID: 28951472 Cisplatin enhances kidney injury molecule-1 (Kim-1) gene expression in kidney S3 cells. PMID: 27591740 By preventing ERK1/2 phosphorylation following renal injury, STAT3 phosphorylation is decreased, leading to less phosphorylated STAT3 within the nucleus, and subsequently less KIM-1 mRNA increases post injury PMID: 29074644 Study used a previously described highly mobile membrane mimetic membrane in combination with a conventional lipid bilayer model to generate a membrane-bound configuration of Tim1 in silico, identified two possible states for a membrane-bound form of Tim1. PMID: 28978444 Our data reveal a previously unknown role for Galpha12 in regulating efferocytosis and that renal tubular epithelial cells require KIM-1 to mediate this process. PMID: 26697979 Urinary L-FABP, NGAL, Kim-1 and albumin levels increased during the acute phase of kidney injury and were significantly correlated with the degree of tubulointerstitial fibrosis during the chronic phase. These markers could detect higher risk of progression to CKD. PMID: 27028054 Blockade of Tim-1 changes Th1/Th2 balance and reduces circulating regulatory T cells to enhance atherosclerosis in LDL receptor knockout mice. PMID: 26821944 Our results suggest that KIM-1 is an endogenous protective mechanism against renal ischemia-reperfusion injury PMID: 25759266 data suggest that TIM-1 signaling plays a direct role in Breg maintenance and induction both under physiological conditions (in response to ACs) and in response to therapy through TIM-1 ligation PMID: 25645598 Deletion of the mucin domain impaired KIM-1-mediated phagocytic function, resulting in increased proinflammatory cytokine production, decreased antiinflammatory growth factor secretion by proximal epithelial cells, and an increase in tissue macrophages. PMID: 25751064 Tim-1 is critical for maintaining self-tolerance by regulating IL-10 production in Bregs PMID: 25582854 blood biomarker that specifically reflects acute and chronic kidney injury PMID: 24904085 Tim-1 expression was lower in a herpes simplex virus-induced Behcet's disease (BD) mouse model compared to that in asymptomatic BD normal (BDN) mice. PMID: 24453431 Tim-1-Fc protects cardiac grafts from chronic rejection by suppressing CD4 Th17 development and functionality. PMID: 24551271 KIM-1 shedding is accelerated by worsening renal cellular injury, and excess soluble KIM-1 competitively inhibits efferocytosis. PMID: 24829508 Endogenous Tim-1 promotes severe systemic autoimmunity and renal disease MRL-Fas(lpr) mice. PMID: 24623145 a major P-selectin ligand with a role in T cell trafficking during inflammatory responses and the induction of autoimmune disease PMID: 24703780 Sustained KIM-1 expression promotes kidney fibrosis and provides a link between acute and recurrent injury with progressive chronic kidney disease. PMID: 23979159 We defined a novel pathway in which TIM-1, a receptor for phosphatidylserine expressed by apoptotic cells, drives the development of asthma by sensing and responding to injured and apoptotic airway epithelial cells. PMID: 23672783 TIM-1 expressing CD4 T cells are required in the mechanism of innate immune-mediated hepatic IRI in OLTs PMID: 23137033 Tim-1 plays a critical role in maintaining suppressive regulatory B-cell function, while it's mucin domain plays an unexpected role in regulating Breg function and maintaining self-tolerance. PMID: 22773818 Endogenous Tim-1 promotes Th1 and Th17 nephritogenic immune responses and its neutralization reduces renal injury while limiting inflammation in cell-mediated glomerulonephritis. PMID: 22205357 Tim-1 functions in pathways that suppress recruitment of inflammatory cells into the airways and the generation or activity of CD4+ T cells. PMID: 22144095 Tim-1 expression declined in Helicobacter pylori infection. PMID: 21923683 Data show that in vivo in mice, TIM-1 is predominantly expressed on B rather than T cells, and is an inclusive marker for IL-10+ Bregs that can be induced by TIM-1 ligation. PMID: 21821911 Tim1 and Tim3 are not essential for the induction of the type-2 response in lung allergy. PMID: 21470319 mAb stimulation on dendritic cells regulates the balance between effector and regulatory T cells and induces Experimental autoimmune encephalitis PMID: 21469101 The TIM-1:TIM-4 pathway enhances injury after renal ischemia-reperfusion injury and may be a therapeutic target. PMID: 21355054 These results suggest that TIM-1 signaling in B cells augments antibody production by enhancing B cell proliferation and differentiation. PMID: 21303660 results suggest that TIM-1 serves as a pattern recognition receptor on NKT cells that senses PtdSer on apoptotic cells as a damage-associated molecular pattern PMID: 20889552 Tim-1 is induced on germinal centre B cells through BCR signaling. PMID: 20518819 TIM1 is an endogenous ligand for LMIR5. PMID: 20566714 Late upregulation of KIM-1 and NGAL could be a useful marker for sustained renal injury after acute kidney injury. PMID: 20181666 TIM1 costimulation on hepatic natural killer T cells enhances cellular production of interleukin (IL)-4 while inhibiting the production of interferon (IFN)-gamma. PMID: 20220086 TIM-1 regulates not only T for the role of cell activation but may also affect macrophage function in the local inflammation response. PMID: 20091883 protective effects of hepatitis A virus on atopic disease depends on a common TIM-1 allele PMID: 14534576 TIM-1 is a molecule that costimulates T cell activation PMID: 15793575 TIM-1-TIM-4 interaction is involved in regulating T cell proliferation PMID: 15793576 TIM-1 is expressed on CD4+ T cells of the lung-draining lymph nodes after intranasal immunization and plays a role in T cell activation and differentiation. PMID: 16284246 These results explain the divergent immune functions described for the murine receptors and the role of TIM-1 as a cell adhesion receptor in renal regeneration and cancer. PMID: 17363299 The Ig domains of murine TIM1, 3 and 4 display calcium-dependent binding to carbohydrate ligands expressed by murine splenocytes and non-murine cell lines. Both homo- and heterotypic interactions were observed for binding between the TIM proteins. PMID: 17513880 Tim-1 regulates T cell responses and can alter T cell function depending on the affinity/avidity with which it is engaged. PMID: 17606630 In vivo abrogation of TIM-4 or its cognate ligand TIM-1 by using a polyclonal antibody remarkably dampened Th2 differentiation and intestinal allergy. TIM-4 as a novel molecule critically required for the development of intestinal allergy. PMID: 17915221 Ligation of Tim-1 in vitro effectively deprogrammed Tregs and thus produced Tregs unable to control T cell responses. PMID: 18079964 TIM-4 and TIM-1 are immunologically restricted members of the group of receptors whose recognition of PS is critical for the efficient clearance of apoptotic cells and prevention of autoimmunity. PMID: 18082433 These studies define previously unknown functions of TIM-1 in regulating alloimmune responses in vivo . PMID: 18172549 These results demonstrate that TIM-1 is significantly increased in pulmonary tissues and PBMCs in asthmatic mice after ovalbumin challenge, and that the production of TIM-1 as well as GATA-3 is upregulated in the spleen of asthmatic mice. PMID: 18234236 Ligation of Tim-1 in the presence of mature dendritic cells triggers polyclonal T cell activation. PMID: 19155484 Results demonstrate that targeting T cell Ig and mucin domain-1 (Tim-1) with anti-Tim-1 overcomes transplantation tolerance resistance. PMID: 19528638

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Mouse TIM-1 Protein (C-6His)

Recombinant Mouse TIM-1 Protein (C-6His)

Product Overview

Target Details

FAQs