Recombinant Mouse TCTP Protein

Name: Recombinant Mouse TCTP Protein
Brand: Beta LifeScience
SKU: BLA-10109P
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Host Species	Mouse
Accession	P63028
Synonym	FLJ27337 Fortilin Histamine releasing factor Histamine-releasing factor HRF Immunoglobulin E-dependent p02 p23 TCTP TCTP_HUMAN tpt1 Translationally controlled tumor protein Translationally-controlled tumor protein Tumor protein translationally controlled 1 tumor protein, translationally-controlled 1
Description	Recombinant Mouse TCTP Protein was expressed in E.coli. It is a Full length protein
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSH MGSHMMIIYR DLISHDELFS DIYKIREIAD GLCLEVEGKM VSRTEGAIDD SLIGGNASAE GPEGEGTESTVVTGVDIVMN HHLQETSFTK EAYKKYIKDY MKSLKGKLEE QKPERVKPFM TGAAEQIKHI LANFNNYQFF IGENMNPDGM VALLDYREDGVTPFMIFFKD GLEMEKC
Molecular Weight	22 kDa including tags
Purity	>90% SDS-PAGE.Purified using conventional chromatography techniques.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Liquid Solution
Stability	The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution	See related COA
Unit Definition	For Research Use Only
Storage Buffer	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Target Details

Target Function	Involved in calcium binding and microtubule stabilization.
Subcellular Location	Cytoplasm.
Protein Families	TCTP family
Database References	KEGG: mmu:22070 STRING: 10090.ENSMUSP00000106519 UniGene: Mm.297482

Gene Functions References

The biological effects of Na,K-ATPase and TCTP interaction are reviewed. PMID: 29867020
that TPT1 knockdown altered the BECN1 interactome, a representative MTOR-independent pathway, to stimulate autophagosome formation PMID: 28409693
The first crystal structure of murine HRF to 4.0A resolution, reveals a conserved fold. A model for the role of murine HRF in mast cell activation was created based on the human HRF structure. PMID: 29216544
Fortilin directly interacts with the cytoplasmic domain of IRE1alpha, inhibits both kinase and endoribonuclease (RNase) activities of this stress sensor, and protects cells against apoptotic cell death at both cellular and whole animal levels. PMID: 28550308
TCTP-mediated spindle dynamics play a key role in maintaining oocyte quality during postovulatory aging. Overexpression of TCTP is sufficient to prevent aging-associated abnormalities in mouse oocytes. PMID: 28476647
Leads to the enhanced and previously un-described interaction of RACK1 and TCTP. PMID: 27816562
These data suggest the presence of a novel oxidative-stress-handling pathway where the anti-p53 molecule fortilin augments the peroxidase PRX1 by protecting it against degradation and inactivation of the enzyme. PMID: 26726832
results demonstrate that TCTP is a microtubule-associating protein required to regulate spindle microtubule dynamics during meiotic maturation in mouse oocytes. PMID: 26802898
TCTP over-expression-induced RhoA activation indicates that silencing of cellular TCTP may offer therapeutic approaches for the regulation of hypertension by inhibiting RhoA/Rho kinase pathway and modulating vascular contractility. PMID: 24918292
osteoclastogenic activity of translationally-controlled tumor protein (TCTP) with reciprocal repression of p21 PMID: 25263704
Fortilin, induced in the proatherosclerotic microenvironment in macrophages, protects macrophages against Bax-induced apoptosis, allows them to propagate, and accelerates atherosclerosis. PMID: 24043250
Overexpression of translationally controlled tumor protein increases the severity of atherosclerosis and hypertension in apolipoprotein E knock-out mice PMID: 22415346
These findings suggest that TCTP might play an important role in the process of podocyte hypertrophy under diabetic conditions via the regulation of mTORC1 activity and the induction of cell-cycle arrest. PMID: 22311416
The TCTP downregulates the Oct4 expression by binding the Sf1 site of Oct4 promoter in mouse pluripotent cells. PMID: 22281008
TCTP may play a significant role in embryo implantation in mice PMID: 21865235
knockdown of Tctp resulted in increased amounts of P53 accompanied by a 50% reduction in the efficiency of tumor mammosphere formation PMID: 22157679
Identified a subset of IgE and IgG antibodies as TCTP(HRF)-interacting molecules in vitro. HRF was able to dimerize and bind to Igs via interactions of its N-terminal and internal regions with the Fab region of Igs. PMID: 22133880
Data indicate that Hprt, Rpl13a and Tpt1 are a set of stably expressed reference genes for accurate gene expression normalization in myocardial infarction studies in mice. PMID: 21858224
The Tpt1-Npm1 complex as a novel marker for highly proliferating cells, and offer further insight into the network that controls cellular fate. PMID: 20505363
Results imply that TCTP levels influence the sensitivity of beta cells to apoptosis PMID: 21063673
TCTP levels influence sensitivity to apoptosis PMID: 19901967
Plk phosphorylates TCTP on 2 Ser residues in vitro & cofractionates with the majority of kinase activity toward TCTP in mitotic cell lysates. Phosphorylation decreases its microtubule-stabilizing activity & promotes increased MT dynamics after metaphase. PMID: 12167714
seven putative Tpt1 genes with different chromosomal localizations were identified; analysis of sequence, genomic organization, and in vitro promoter activity. PMID: 12782126
TCTP stabilizes Mcl-1 through interfering with Mcl-1's degradation by the ubiquitin-dependent proteasome degradation pathway and modulates its antiapoptotic activity. PMID: 15798198
an N-terminal region of a cytosolic protein, TCTP, is required for its binding to Bcl-xL and for its antiapoptotic activity PMID: 15870695
These results suggest that despite that TCTP is widely expressed in many tissues or cell types, it appears to regulate cell proliferation and survival in a tissue- or cell type-specific manner. PMID: 17475776
Fortilin is an intracellular Ca2+ scavenger, protecting cells against Ca2+-dependent apoptosis by binding and sequestering divalent calcium ions (Ca2+) from the downstream Ca2+-dependent apoptotic pathways. PMID: 17705784
TCTP antisense expression in CD25(high) regulatory T cells aggravates cuff-injured vascular inflammation PMID: 18789801
In early phase of development, fortilin functions as an inhibitor of the bone morphogenetic protein pathway. PMID: 19364479
Study demonstrated that TCTP acts as a cataractogenic factor through the repression of Na,K-ATPase activity and calcium mobilization in lens epithelial cells. PMID: 19479337
Proton pump inhibitors exert anti-allergic effects by reducing TCTP secretion PMID: 19484128
TCTP plays a very modest role in thymocyte development, but is critical for peripheral T cell maintenance and T cell receptor (TCR)-mediated cell proliferation. PMID: 19605695

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.