Recombinant Mouse Protein Wnt-5A (WNT5A) Protein (His)

Name: Recombinant Mouse Protein Wnt-5A (WNT5A) Protein (His)
Brand: Beta LifeScience
SKU: BLC-00103P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: High-quality cytokines for advanced research, High-quality recombinant proteins

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Product Overview

Description	Recombinant Mouse Protein Wnt-5A (WNT5A) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Activity	Not tested.
Uniprotkb	P22725
Target Symbol	WNT5A
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	C-6His
Target Protein Sequence	IIGAQPLCSQLAGLSQGQKKLCHLYQDHMQYIGEGAKTGIKECQYQFRHRRWNCSTVDNTSVFGRVMQIGSRETAFTYAVSAAGVVNAMSRACREGELSTCGCSRAARPKDLPRDWLWGGCGDNIDYGYRFAKEFVDARERERIHAKGSYESARILMNLHNNEAGRRTVYNLADVACKCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMRLNSRGKLVQVNSRFNSPTTQDLVYIDPSPDYCVRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTVQTERCHCKFHWCCYVKCKKCTEIVDQFVCK
Expression Range	62-380aa
Protein Length	Full Length of Mature Protein
Mol. Weight	42.7 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
Subcellular Location	Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families	Wnt family
Database References	KEGG: mmu:22418 STRING: 10090.ENSMUSP00000064878 UniGene: PMID: 28469167 Activating effect of WNT5A regulated bone formation in response to hindlimb unloading in mice, and pretreatment with WNT5A partly rescued the osteoporosis caused by mechanical unloading. PMID: 30007964 calcineurin is a key regulator of Wnt5a-induced AQP2 activation without affecting intracellular cAMP level and PKA activity. PMID: 27892464 The findings demonstrate that induction of Vangl protein phosphorylation plays an essential role in transducing Wnt5a signaling to establish Planar cell polarity (PCP) in mammalian development, suggesting a phosphorylation-regulated "Vangl activity gradient" model in addition to the well-documented "Fz activity gradient" model in Wnt/PCP signaling. PMID: 29056748 Post-mitotic filopodial "pathfinding" is guided by mesenchymal WNT5A. Without WNT5A, some cells fail to tether basally and undergo apoptosis, leading to a shortened midgut. PMID: 30016620 We demonstrate with genetic evidence that the Wnt5a gradient acts as a global cue that is instructive in establishing planar cell polarity (PCP) in the limb mesenchyme, and that Wnt5a also plays a permissive role to allow Fgf4 and Fgf8 signaling to orient PCP. PMID: 29615464 Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development. PMID: 27561676 These data suggest that Wnt5a modulates the development of arthritis by promoting inflammation and osteoclast fusion, and provide the first mouse genetic evidence of a role for endogenous Wnt5a in autoimmune disease. PMID: 28724439 Here, we show that deletion of a single Wnt family member, Wnt5a, is sufficient to elicit profound disruptions in synaptic plasticity, structural maintenance, and learning and memory in adult mice, identifying the importance of this particular noncanonical Wnt in later-life functions. PMID: 28069946 Wnt5a promotes IL-6 and TIMP-1 release from mouse cardiac fibroblasts. PMID: 28357477 bivalent histone codes during odontogenic differentiation PMID: 28880717 WNT5A promoted spermatogonial stem cells activity both in vitro and in vivo. PMID: 27509137 results indicate that Wnt5a signaling restricts infection by antimony drug-sensitive and -resistant Leishmania donovani strains, at least in part by prohibiting parasite niche formation within host macrophages PMID: 28659356 During mouse embryonic stem cell (ESC) in vitro osteogenesis, the noncanonical WNT5a is expressed early on. PMID: 26956615 This study shows that Wnt5a is not required for development of the renal medulla and that loss of the renal medullary region in the Wnt5a-deleted kidney is caused by an abnormal ureter-bladder connection. PMID: 27923152 WNT5A in the bone marrow niche is required to regenerate hematopoietic stem cells and leukemic cells with functional ability to rearrange the actin cytoskeleton and engraft successfully. PMID: 27998927 This study describes the localization and functional role of WNT-5A in human and mouse fibrotic livers. Hepatic WNT-5A expression parallels collagen type I expression. In vivo and in vitro, the myofibroblasts were identified as the key hepatic cells producing WNT-5A. WNT-5A is under control of TGF-beta and its activities are primarily profibrotic. PMID: 28057611 we confirmed the requirement of Wnt5a in the deferoxamine-mediated osteoblast-promoting effects by analyzing the matrix mineralization of Wnt5a-deficient cells. The promoting effect of deferoxamine on matrix mineralization in wild-type cells was completely abolished in Wnt5a(-/-) cells. PMID: 27540134 opposing gradients of Wnt5a and Wnt5b and of their Sfrp inhibitors, together with intercellular signaling via planar cell polarity proteins, polarize node cells along the anterior-posterior axis for breaking of left-right symmetry. PMID: 28292423 Wnt5a is responsible for maintaining effective communication between the stromal and epithelial compartments partly through the action of luminal and stromal secreted factors, Fgf10 and Ihh. PMID: 27965056 findings reveal that FZD9 and heterotrimeric G proteins regulate Wnt-5a signaling and dendritic spines in cultured hippocampal neurons. PMID: 27402827 Here we show that an evolutionarily conserved genomic region named AS3_9 comprises three TEs (AmnSINE1, X6b_DNA and MER117), inserted side-by-side, and functions as a distal enhancer for wnt5a expression during morphogenesis of the mammalian secondary palate. PMID: 27741242 we here reported that Wnt5a mediated activation of canonical Wnt signaling pathway might contribute to the orthodontic force induced bone remodeling. PMID: 27456852 Wnt5a deficiency can regulate inflammatory cytokine secretion, polarization, and apoptosis in MTB-infected macrophage PMID: 27828711 These results showed that Wnt5a could promote the maturation of mast cells via the canonical Wnt signaling pathway and provide important insights into the molecular mechanisms underlying the differentiation of mast cells PMID: 27843938 the CaMKK-CaMKIalpha cascade is required for the axonal growth effect of Wnt5a during neuronal polarization. PMID: 26772170 indicate that restricted expression of Wnt5a in the caudal SpM is essential for normal OFT morphogenesis, and uncover a novel function of spatially regulated cell cohesion by Wnt5a in driving the deployment of SHF cells from the SpM into the OFT PMID: 26916252 Findings indicate wingless-related MMTV integration site 5A protein (WNT5a) as a key regulator of follicle development and gonadotropin responsiveness. PMID: 26667040 The results in a potent increase in odontoblastic cell differentiation, indicating the unique involvement of Atg5, autophagy and Wnt5 signaling in CS/BMP-4-induced differentiation of ES cells into odontoblast-like cells, at a relatively early stage. PMID: 26806855 the Wnt5a/Frizzled-2 axis suppresses beta-catenin signaling in hepatocytes in an autocrine manner, thereby contributing to timely conclusion of the liver regeneration process PMID: 26100214 effects on hypoxic pulmonary hypertension by regulating pulmonary vascular remodeling and right ventricular hypertrophy via reduction in beta-catenin/cyclin D1 signaling PMID: 25956683 Wnt5a regulates the pathogenesis of gp120-induced pain. PMID: 25840108 Study identified a novel Wnt5a/Fzd4 signaling pathway that contributes to the regulation of dendrite morphogenesis PMID: 25424568 The Wnt5a-Ror2 axis promotes the signaling circuit between interleukin-12 and interferon-gamma in colitis PMID: 26030277 study presents the finding that activation of the NF-kappaB pathway by Porphyromonas endodontalis LPS is important for Wnt5a expression in osteoblasts, and that TRIB3 may modulate the sustained expression of Wnt5a in osteoblasts stimulated by P. endodontalis PMID: 25601649 Wnt5a and its receptors are sorted to their correct destination by different mechanisms and that the basolateral secretion of Wnt5a is necessary for apical lumen formation in the epithelial cyst. PMID: 25593127 In Wnt5a null mice, second heart field (SHF) progenitors are trapped in the SHF and fail to be deployed to the outflow tract (OFT) efficiently, resulting in a reduction in the inferior OFT myocardial wall and subpulmonary myocardium. PMID: 25410658 Wnt5a abrogated the inhibitory effects of Wnt16 on Rankl-induced osteoclastogenesis PMID: 26093292 Therefore, Wnt5a-induced noncanonical signaling cooperates with Wnt/beta-catenin signaling to achieve proper bone formation. PMID: 24670389 findings demonstrate, for the first time, that Wnt5a reduces the accumulation of cholesterol in lipid-loaded cells by regulating the mRNA expression of Caveolin-1 and ABCA1, which are involved in reverse cholesterol transport. PMID: 24827906 There is an important role of Wnt5a in kidney development. Disrupted Wnt5a results in kidney cysts in zebrafish and pleiotropic abnormal kidney development in mice. PMID: 25412793 Skin of Wnt5a TG mice was not psoriatic, but characterized with normal proliferation and homeostasis of epidermis. Instead, the TG mice displayed impaired hair follicle transition from telogen to anagen, likely due to impaired canonical Wnt signalling. PMID: 25219536 Non-canonical Wnt5a/Ror2 signaling regulates kidney morphogenesis by controlling intermediate mesoderm extension. PMID: 25082826 Adenovirus-mediated Wnt5a silencing in vivo attenuates the development of atherosclerotic disease. PMID: 25050997 Wnt5a through Wnt/JNK signaling alone or both Wnt/JNK and Wnt/PKC signaling promoted the differentiation of mMSCs into type II alveolar epithelial cells and the migration of mMSCs. PMID: 24658098 Wnt5a regulates HSC quiescence and hematopoietic repopulation through the Ryk receptor and that this process is mediated by suppression of reactive oxygen species. PMID: 23939973 The activation of Wnt5a-Ror2 signaling in epithelial cells undergoing epithelial-to-mesenchymal transition (EMT) may play an important role in disrupting TBM via MMP-2 induction during renal fibrosis. PMID: 23755735 The results indicate an important role of Wnt5a-Ror2 signaling in morphogenesis of the metanephric mesenchyme to ensure proper epithelial tubular formation of the ureteric bud required for kidney development. PMID: 24891614 Wnt5a-mediated Ryk activation triggers divergent responses in callosal axons and dendrites in the in vitro context. PMID: 24471468 Studied expression of Wnt family member 5A in murine hair cycle and its inhibitory effects on follicle in vivo. PMID: 24507677

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.