Recombinant Mouse Protein Dek (DEK) Protein (His)

Name: Recombinant Mouse Protein Dek (DEK) Protein (His)
Brand: Beta LifeScience
SKU: BLC-03754P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins, Recombinant proteins fall special offers - full-length proteins

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Product Overview

Description	Recombinant Mouse Protein Dek (DEK) Protein (His) is produced by our Baculovirus expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q7TNV0
Target Symbol	DEK
Synonyms	DekProtein DEK
Species	Mus musculus (Mouse)
Expression System	Baculovirus
Tag	N-10His
Target Protein Sequence	MSAAAAPAAEGEDAPVPPSSEKEPEMPGPREESEEEEEDDEDDDEEDEEEEKEKSLIVEGKREKKKVERLTMQVSSLQREPFTVTQGKGQKLCEIERIHFFLSKKKPDELRNLHKLLYNRPGTVSSLKKNVGQFSGFPFEKGSTQYKKKEEMLKKFRNAMLKSICEVLDLERSGVNSELVKRILNFLMHPKPSGKPLPKSKKSSSKGSKKERNSSGTTRKSKQTKCPEILSDESSSDEDEKKNKEESSEDEEKESEEEQPPKKTSKKEKAKQKATAKSKKSVKSANVKKADSSTTKKNQKSSKKESESEDSSDDEPLIKKLKKPPTDEELKETVKKLLADANLEEVTMKQICKEVYENYPAYDLTERKDFIKTTVKELIS
Expression Range	1-380aa
Protein Length	Full Length
Mol. Weight	45.7 kDa
Research Area	Epigenetics And Nuclear Signaling
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Involved in chromatin organization.
Subcellular Location	Nucleus.
Database References	KEGG: mmu:110052 STRING: 10090.ENSMUSP00000021807 UniGene: PMID: 28317934 DEK is crucial to the development of arthritis in mouse models, thus making it an appropriate target for aptamer-based therapy. PMID: 28165452 DEK was expressed throughout the brain in both sexes, including the medial prefrontal cortex (prelimbic, infralimbic and dorsal peduncular). DEK was also abundant in all amygdalar subdivisions (basolateral, central and medial) and in the hippocampus including the CA1, CA2, CA3, dentate gyrus (DG), ventral subiculum and entorhinal cortex. PMID: 29175155 Dek contributes to esophageal squamous cell carcinoma tumorigenesis at least partially through keratinocyte intrinsic pathways which promote cellular and tumor growth. PMID: 29538372 Data suggests that DEK participates in the process of embryo implantation and might play a role in influencing stromal cell decidualization through mediating cell proliferation, apoptosis and DNA damage. PMID: 29109007 DEK expression during normal hematopoiesis did reveal a relationship with specific cell types implicating a distinct function during myeloid differentiation PMID: 25128083 Studies demonstrate that DEK overexpression, due in part to Ron receptor activation, drives breast cancer progression through the induction of Wnt/beta-catenin signaling. PMID: 24954505 Results highlight a novel function of DEK as a guardian of chromatin by restricting widespread histone access, and in particular by modulating differential H3.3 loading in specific chromatin areas. PMID: 25049225 data demonstrate that DEK stimulates HNSCC cellular growth and identify DeltaNp63 as a novel DEK effector PMID: 24608431 DEK has potent effects on HSCs, HPCs, and hematopoiesis, information of biological and potential clinical interest. PMID: 21943234 The hallmark of translocation in acutte myeloid leukemiaa is the expression of the DEK/CAN fusion protein. PMID: 20827285 DEK is a neuroblastoma tumor antigen. PMID: 17397536 Our conclusion is that DEK could serve as an architectural protein at the promoter or enhancer sites of a subfraction of human genes. PMID: 17524367 Overexpression of the cellular DEK protein promotes epithelial transformation in vitro and in vivo. PMID: 19223548

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.