Recombinant Mouse MIP-1 alpha / SCYA3 Protein

Beta LifeScience SKU/CAT #: BLPSN-3371

Recombinant Mouse MIP-1 alpha / SCYA3 Protein

Beta LifeScience SKU/CAT #: BLPSN-3371
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Product Overview

Tag N/A
Host Species Mouse
Accession P10855
Synonym AI323804, G0S19-1, LD78alpha, MIP-1alpha, MIP1-(a), MIP1-alpha, Mip1a, Scya3
Background CCL3 is a cytokine belonging to the CC chemokine family. Chemokines are a family of structurally related leukocyte chemoattractant cytokines that play a central role during immunoregulatory and inflammation processes. All chemokines contain four conserved cysteines linked by disulfide bonds, and two major subfamilies, namely CXC and CC, are defined on the basis of the first two cysteines which are separated by one amino acid or are adjacent. CCL3 is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description A DNA sequence encoding the mouse CCL3 (P10855) (Ala24-Ala92) was expressed and purified.
Source E.coli
Predicted N Terminal Met
AA Sequence Ala24-Ala92
Molecular Weight The recombinant mouse CCL3consists of 70 a.a. and predicts a molecular mass of 8.01 KDa. It migrates as an approximately 8-14 KDa band in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin Please contact us for more information.
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile 30% acetonitrile 0.1%TFA.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.
Subcellular Location Secreted.
Protein Families Intercrine beta (chemokine CC) family
Database References
Tissue Specificity Expressed in lung, spleen, and pancreas.

Gene Functions References

  1. During early remission from methamphetamine (MA), model mice exhibited increased anxiety-like behavior and reduced expression of chemokine (C-C motif) ligand 3 (ccl3) in the hippocampus relative to saline-treated mice. Human adults actively dependent on MA and those in early remission had elevated symptoms of anxiety as well as reductions in plasma levels of CCL3, relative to adults with no history of MA abuse. PMID: 29402784
  2. miR-125b could play an important role in inflammatory injury of chondrogenic cells and miR-125b affected inflammatory injury of ATDC5 cells via regulating expression of MIP-1alpha and regulating NF-kappaB and JNK signaling pathways. PMID: 29550827
  3. Leukemogenic effects of the Ptpn11E76K/+mutation in the stem-cell microenvironment is abolished in Ptpn11E76K/+Prx1-Cre+CCL3-/- mice, indicating that CCL3 is important for leukemic transformation. PMID: 28751523
  4. Parenchymal polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC), have a positive correlation with IL1a, IL8, CXCL5, and Mip-1a, suggesting they may attract PMN-MDSC into the tumor PMID: 27799249
  5. CCL3 plays a significant role in the clearance of infection and resolution of otitis media inflammation and contributes to mucosal host defense of the nasopharyngeal niche, a reservoir for middle ear and upper respiratory infections. PMID: 28847849
  6. Cytokine array analysis demonstrated that the chemokine CCL3 was elevated in the plasma of AML mice and patients. CCL3 inhibited erythroid differentiation of hematopoietic stem cells, common myeloid progenitors and especially megakaryocytic-erythroid progenitors. PMID: 27109512
  7. MCP chemokines are activated in peripheral tissues of breast cancer-bearing mice, by a mechanism that involves breast cancer cell-derived Ccl3. PMID: 28041977
  8. These observations suggest that intra-BM basophil expansion can favor leukemia-tropic hematopoiesis in myeloid leukemia by providing CCL3, a potent inhibitor of normal hematopoiesis and that basophil-derived CCL3 may be a novel target molecule for the treatment of myeloid leukemia. PMID: 27006388
  9. A marked increase of CCL3 mRNA expression was observed in inflamed paws, with CCL3 protein detected in neutrophils and macrophages by immunohistochemical experiments. PMID: 26663750
  10. CCL2/CCL3 double-mutant animals are viable, fertile, and do not present with gross abnormalities. Cuprizone increased CCL3 expression in wild-type but not mutant mice. Cuprizone-induced demyelination, oligodendrocyte loss, and astrogliosis were significantly ameliorated in the cortex but not corpus callosum of chemokine-deficient animals. PMID: 25663168
  11. these data suggest that the chemokine CCL3 is an hippocampal neuromodulator able to regulate synaptic plasticity mechanisms involved in learning and memory functions. PMID: 26511387
  12. Ccl3 mRNAs increased within 5 h after injury in mouse cortical slices. PMID: 25895671
  13. Previous exposure to noninjurious ventilation induces a state of tolerance to ventilator-induced lung injury. Down regulation of the chemokine gene Ccl3 could be the mechanism responsible for this effect. PMID: 26472813
  14. the tissue fibrinolytic system contributes to the induction of macrophage recruitment and CCL3 at the bone injury site, thereby, leading to the enhancement of the repair process. PMID: 25893677
  15. APOE varepsilon4 genotype specifically modulates astrocyte secretion of potent microglial chemotactic agents, including CCL3 PMID: 25092803
  16. In experimental autoimmune encephalomyelitis there was a significant increase in activation of astrocytes with increased MIP-1alpha expression. PMID: 24989845
  17. Negative modulation of PGE2 signaling reduced infection-induced anti-inflammatory cytokine polarization and enhanced inflammatory chemokines, CCL3 and CCL5. PMID: 25049356
  18. Granzyme M regulates the release of natural killer cell MIP-1alpha to initiate innate immune responses. PMID: 24625974
  19. CCL3-CCR5-mediated fibroblast accumulation may be required, in addition to leukocyte infiltration, to induce full-blown colitis-associated carcinogenesis. PMID: 24510316
  20. CCL3 production by microglial cells modulates disease severity in murine models of retinal degeneration. PMID: 24639355
  21. results define the chemokine CCL3 as a mediator of experimental liver fibrosis. Thus, therapeutic modulation of CCL3 might be a promising target for chronic liver diseases PMID: 23799074
  22. BCR-ABL(+)lineage(-)c-kit(-) immature leukemia cells produced high levels of an inflammatory chemokine, MIP-1alpha/CCL3, which promoted the development of chronic myeloid leukemia. PMID: 24166712
  23. The CCR5/MIP-1alpha axis may contribute to migration of infected cells to the brain and consequently affect the pathogenesis during Rocio virus infection. PMID: 24080631
  24. Data indicate that Bifidobacterium infantis feeding to Salmonella-infected mice significantly reduced Peyer's patch MIP-1alpha and MIP-1beta secretion. PMID: 23648818
  25. IFN-gamma and NOS2 repress CCL3 and CCL4 in vivo upon S. typhimurium infection of mice also repress CCL3 and CCL4 during S. typhimurium infection in bone marrow-derived macrophages. PMID: 23431040
  26. Under conditions of acute inflammation leukocyte-derived CCL3 can induce neutrophil chemotaxis toward the atherosclerotic plaque, thereby accelerating lesion formation. PMID: 23288165
  27. findings show that CCR1 is pivotal for bone remodeling induced by mechanical loading during orthodontic tooth movement and these actions depend, at least in part, on CCL3 PMID: 23059626
  28. Hyperocclusion induced compensatory CCL3 expression; promoted osteoclastogenesis to counterbalance deficient CCL2/CCR2 signaling. co-expression of CCL3 with CCL2 may precipitate synergistic, MS-dependent alveolar destruction during occlusal traumatism. PMID: 23143790
  29. The kinetics and extent of microglia and astrocyte activation during early and late cuprizone-induced demyelination in the white matter tract corpus callosum and the telencephalic gray matter were related to the expression of the Ccl2 and Ccl3. PMID: 22528463
  30. Expression of ALDH1A2, BEX2, EGR2, CCL3 and PLAU are upregulated in Toxoplasma gondii susceptible C57BL/6 mice. PMID: 22451728
  31. role for CCL3 in lipid metabolism in hyperlipidemic mice following hematopoietic reconstitution. PMID: 22359597
  32. In a murine carotid aneurysm model of vascular inflammatory repair, monocyte chemotactic protein (MCP)-1-mediated tissue in-growth occurs via a MIP-1alpha- and MIP-2-regulated pathway. PMID: 22007074
  33. our data indicate that the interaction between TTP and CCL3 mRNA plays an important role in modulating localized inflammatory processes in tissues that are dissociated from the systemic manifestations of chronic inflammation. PMID: 21784977
  34. The selective interaction of CCL3 with its receptor, CCR1, is critical for radiation-induced lung inflammation and fibrosis, and these conditions can be largely prevented by a small molecule inhibitor of CCR1 PMID: 20870892
  35. Absence of TLR3 in skin-injured mice leads to defective recruitment of neutrophils and macrophages, in association with decreased expression of the chemokines MIP-2/CXCL2, MIP-1alpha/CCL3, and MCP-1/CCL2 in the wound. PMID: 21317384
  36. MIP-1alpha and interferon-gamma production by CD1d-restricted natural killer (NK)T cells contributes to immune complex-induced acute lung injury. PMID: 21191075
  37. Maternal cells can migrate through the blood-placenta barrier even in the absence of fetal CCL3 in mice. PMID: 20567093
  38. This work demonstrates that the development of F. hepatica is not affected by the absence of CCL3. PMID: 20643506
  39. These results suggest that peripheral nerve injury elicits the up-regulation of spinal MIP-1alpha and CCR1 to participate in neuropathic pain. PMID: 20692319
  40. results suggest a close association between the MIP-1alpha response and the Th1-type T-cell responses in chlamydial lung infections PMID: 20622889
  41. Results show that CCL3 regulates the balance of T cell populations in the lung and can alter the outcome of RSV infection. PMID: 20195359
  42. In conclusion, we propose a novel critical molecule MIP-1alpha derived from macrophages and Schwann cells that appears to play a crucial role in the development of neuropathic pain induced by PSL. PMID: 20223588
  43. OGT could be a co-regulatory subunit shared by functionally distinct complexes supporting epigenetic regulation of MIP-1alpha gene promoter. PMID: 20206135
  44. Studies demonstrate that CCL3 plays a major role in mediating GVHD, but not graft-versus-leukemia. PMID: 20100934
  45. CCL3 is a potent downstream effector of tumor necrosis factor-mediated inflammation in vitro and in vivo. PMID: 20164426
  46. we identified a novel role for neutrophil-secreted CCL3 in the first wave of dendritic cells recruitment to the site of infection with L. major PMID: 20140197
  47. Data verified that the expression of CCR5 and its ligands, CCL3, CCL4 and CCL5, were increased in the thymus with age. PMID: 20046229
  48. MIP-1a is the key substrate in the lung for matrix metalloproteinase-8 during lipopolysaccharide-mediated acute lung injury in mice. PMID: 20042585
  49. GA exhibits antiinflammatory effects through inhibition of MIP-1alpha in a mouse model of acute P. acnes-induced inflammatory liver injury. PMID: 19897483
  50. MIP-1alpha, MIP-1beta, RANTES, and ATAC/lymphotactin function together with IFN-gamma as type 1 cytokines. PMID: 11972057


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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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