Recombinant Mouse MDC Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3321
Recombinant Mouse MDC Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3321
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Mouse |
Accession | O88430 |
Synonym | ABCD-1, DCBCK, MDC, Scya22 |
Background | Chemokine (C-C motif) ligand 22(ABCD-1 / CCL22)is a kind of CC chemokine which is a family of secreted proteins involved in immunoregulatory and inflammatory processes. The cytokine displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. This ABCD-1 / CCL22 chemokine binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated / effector T lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. ABCD-1 / CCL22 is highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus, and in Langerhans' cell histiocytosis and atopic dermatitis but not in dermatopathic lymphadenopathy. This chemokine is also found in lymph node, appendix, activated monocytes, resting and activated macrophages. This protein is lower expressed in lung and spleen and very weekly expressed in small intestine. |
Description | A DNA sequence encoding the mouse CCL22 (O88430) (Gly26-Ser92) was expressed with a His tag at the N-terminus. |
Source | E.coli |
Predicted N Terminal | His |
AA Sequence | Gly26-Ser92 |
Molecular Weight | The recombinant mouse CCL22 consists of 84 a.a. and predicts a molecular mass of 9.9 KDa. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile 0.1 % TFA, 50 % acetonitrile.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B-lymphocytes with T-cells in immune responses. |
Subcellular Location | Secreted. |
Protein Families | Intercrine beta (chemokine CC) family |
Database References | |
Tissue Specificity | Expressed by activated splenic B-lymphocytes and dendritic cells. Low expression in lung, thymocytes, lymph node, and unstimulated splenic cells. |
Gene Functions References
- the subset of peritoneal CD11b(+)CD169(+) macrophages increased and CCL22 expression level decreased significantly during the DSS-induced colitis PMID: 28466432
- Intratumoral administration of anti-CCL22 antibody inhibited B16F10 melanoma growth. PMID: 28668835
- this study shows that intravenous injection of apoptotic cells induces a subsequent increase in CCL22 expression and CCR4+ Treg cells, which contribute to the maintenance of immune homeostasis at least partially by splenic CD8alpha+ CD103+ dendritic cells PMID: 26868141
- CCL22-specific antibodies reveal that engagement of two distinct binding domains on CCL22 is required for CCR4-mediated function. PMID: 26683175
- CCL22 was localised mainly on the cell surface and or in the cytoplasm. Within sections of omental milky spot micrometastases, CCR4 was recognised on or in gastric cancer cells, constituent cells milky spots, blood cells and blood endothelial cells PMID: 25245466
- results show that the IL-4/CCL22/CCR4 axis is involved in the migration of Tregs to osteolytic lesion sites, and attenuates development of lesions by inhibiting inflammatory migration and the production of proinflammatory and osteoclastogenic mediators PMID: 25264308
- CCL22 has a role in Treg skin homing to suppress depigmentation PMID: 25634358
- CCL22 is a novel mediator of lung inflammation following hemorrhage and resuscitation PMID: 25136780
- The immunomodulatory properties of CCL22 could be harnessed for prevention of graft rejection and type 1 diabetes as well as other autoimmune disorders. PMID: 25740943
- Data indicate macrophage-derived chemokine CCL22 as an adjuvant could enhance the immune protective effect of NTHi-P6 protein vaccine to an extent. PMID: 25200152
- islet expression of CCL22 recruits Tregs and attenuates autoimmune destruction of beta cell PMID: 21737880
- Data show that the presence of the Ccr4 and Ccl22 transcripts were detected in brain slices. PMID: 21177120
- These results suggest that CCL22 functions to regulate development of experimental autoimmune encephalomyelitis through macrophage chemoattraction and effector function. PMID: 20940325
- role of CCL22 for the recruitment of eosinophils during allergic pleurisy PMID: 12629149
- functionally increased expression during Salmonella enterica serovar Typhimurium infection of dendritic cells PMID: 15731072
- Regulatory elements of the CCL22 gene required to mediate a strong and highly specific expression are included in a 4.1-kb promoter region, with the major elements active in dendritic cells and B cells being located in a small proximal 250-bp region. PMID: 15843561
- CCL22 was selectively upregulated in osteoclast-like cells derived from RAW264.7 cells and mouse bone marrow cells upon stimulation with RANKL (receptor activator of nuclear factor-kappaB ligand). PMID: 16821125
- IgE appears to be capable of stimulating basophils to produce MDC in the absence of a specific Ag, which may contribute to IgE-mediated and/or Th2-predominant allergic inflammation. PMID: 18832724
- When isolated, only natural killer (NK)-containing cellular fractions secrete CCL22, and the same fraction isolated from metastatic Lewis lung carcinoma (LLC)-bearing lungs secrete higher levels. PMID: 19234170
- Mice genetically deficient in CCR4 (the receptor for CCL22) show markedly reduced indoleamine dioxygenase (IDO) expression in MLN-DCs and support the involvement of the CCL22/CCR4 axis in IDO induction. PMID: 19843945