Recombinant Mouse Lymphotactin Protein (His Tag)

Name: Recombinant Mouse Lymphotactin Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-3264
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Mouse
Accession	P47993
Synonym	AI661682, ATAC, Lptn, LTN, SCM-1, SCM-1a, Scyc1
Background	This antimicrobial gene encodes a member of the chemokine superfamily. Chemokines function in inflammatory and immunological responses, inducing leukocyte migration and activation. The encoded protein is a member of the C-chemokine subfamily, retaining only two of four cysteines conserved in other chemokines, and is thought to be specifically chemotactic for T cells. This gene and a closely related family member are located on the long arm of chromosome 1. Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description	A DNA sequence encoding the mouse XCL1 (P47993) (Met 1-Gly 114) was expressedï¼Œwith a C-terminal His tag.
Source	Baculovirus-Insect Cells
Predicted N Terminal	Val 22
AA Sequence	Met 1-Gly 114
Molecular Weight	The secreted recombinant mouse XCL1 consists of 103 a.a. and has a calculated molecular mass of 11.5 kDa. It migrates as an approximately 20 kDa band in SDS-PAGE under reducing conditions.
Purity	>80% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4, 10% gly.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Chemotactic activity for lymphocytes but not for monocytes or neutrophils. In thymus, mediates medullary accumulation of thymic dendritic cells and contributes to regulatoy T cell development, playing a role in self-tolerance establishment.
Subcellular Location	Secreted.
Protein Families	Intercrine gamma family
Database References	KEGG: mmu:16963 STRING: 10090.ENSMUSP00000027860 UniGene: Mm.190
Tissue Specificity	Expressed in activated CD8(+) T cells. In the thymus, expressed by medullary thymic epithelial cells.

Gene Functions References

study provides in vitro and in vivo evidence that the interaction between XCL1 and alpha9 integrin has an important role for autoimmune diseases PMID: 28550205
The interaction between XCL1 and XCR1 plays a crucial role in the classical immunology response. PMID: 27387353
Data show that mice lacking either XCL1 or XCR1 exhibit similar specific defects in intestinal T cell populations as XCR1-dendritic cells (DC)-deficient mice. Therefore it is possible that the XCR1-XCL1 axis itself is directly involved in intestinal T cell homeostasis, via a mechanism that might either involve interaction with XCR1+ DCs or be independent of this pathway. PMID: 27005831
T inflammatory memory CD8 T cells participate to antiviral response and generate secondary memory cells with an advantage in XCL1 production PMID: 22528127
XCL1-mediated accumulation of dendritic cells in the thymic medulla contributes to naturally occurring regulatory T cell development and is regulated by Aire. PMID: 21300913
Data report the identification of a new stable molecular assembly formed between the ATAC and Mediator complexes in mouse embryonic stem cells. PMID: 20508642
The expression patterns of XCR1 and XCL1 were conserved in human and mice blood cells, including certain dendritic cell subsets. PMID: 20541533
MIP-1alpha, MIP-1beta, RANTES, and ATAC/lymphotactin function together with IFN-gamma as type 1 cytokines. PMID: 11972057
Adenovirus-mediated intratumoral Lptn gene transfer potentiates the antibody-targeted superantigen therapy of experimental melanoma. PMID: 12226740
In a brain-targeted HIV-1 Tat transgenic model, Tat expression up-regulates XCL1 production not only in Tat-expressing murine astrocytes, but also in monocytes and macrophages/microglia. PMID: 14734774
Lymphotactin gene-modified dendritoma induces T-cell proliferation and strong cytotoxic T lymphocytes reaction against allogenic hepatocellular carcinoma cells. PMID: 17990361
Results describe the role of XCL1 during the course of the immune response to pulmonary M. tuberculosis infection. PMID: 19097989

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.