Recombinant Mouse Lumican Protein (C-6His)

Name: Recombinant Mouse Lumican Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-0714NP
Availability: InStock

BL-0714NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Keratan Sulfate Proteoglycan lumican is produced by our Mammalian expression system and the target gene encoding Gln19-Asn338 is expressed with a 6His tag at the C-terminus.
Accession	P51885
Synonym	Keratan sulfate proteoglycan lumican;KSPG lumican;Lumican
Gene Background	Keratan sulfate proteoglycan lumican, mostly known as lumican, belongs to the SLRP class II subfamily and the small leucine-rich proteoglycan (SLRP) family. Lumican binds to laminin. It has positive regulation of transcription from RNA polymerase II promoter and transforming growth factor beta1 production. Lumican is expressed in a variety of tissues, including skin, artery, lung, cornea, kidney, bone, aorta, and articular cartilage. The abnormal connective tissue phenotype seen in the Lumican null mice shows the importance of the role of Lumican in collagen fibrillogenesis. In addition to the control of collagen fibril assembly, Lumican has been shown to play a role in the regulation of cell proliferation, migration, and adhesion.
Molecular Mass	37.4 KDa
Apmol Mass	40-60 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Subcellular Location	Secreted, extracellular space, extracellular matrix.
Protein Families	Small leucine-rich proteoglycan (SLRP) family, SLRP class II subfamily
Database References	KEGG: mmu:17022 STRING: 10090.ENSMUSP00000040877 UniGene: PMID: 27682997 ectopic mutant lumican (L199P) may induce enlargement of axial lengths and abnormal structures and distributions of collagen fibrils in mouse sclera PMID: 27711221 alternations of lumican might be implicated in the pathogenesis of cardiac fibrosis PMID: 27939890 LUM may coordinate multiple factors of collagen assembly in the murine heart. PMID: 25886697 Lumican and biglycan influence corneal keratocyte lamellipodia organization and are critical in the regulation of stromal collagen fibrillogenesis. PMID: 24447998 Alterations in glycosylated lumican core protein might be implicated in myocardial remodeling and reverse remodeling, with a potential important role for CS/DS GAG chain-synthesizing enzymes. PMID: 23412898 pro-inflammatory cytokine TNF-alpha was comparably low 24 hours after infection, but significantly higher in the Lum(-/-) compared to Lum(+/-) infected corneas by 2-5 days after infection PMID: 23358433 lumican present in the reactive stroma surrounding prostate primary tumors plays a restrictive role on cancer progression PMID: 23399832 To investigate the functional role of lumican in hepatic fibrosis, lumican null (Null) and wild-type (WT) littermates were administered carbon tetrachloride intra-peritoneally. Hepatic fibrosis was greatly reduced in null animals. PMID: 23007134 lumican has a major protective role in gram-negative bacterial infections, a novel function for small leucine-rich repeat proteoglycans. PMID: 22865855 deficiency combined with fibromodulin deficiency manifest several clinical features or Ehlers-Danlos syndrome PMID: 12089156 The results indicate that the cysteine-rich domain of lumican is important in collagen fibrillogenesis and stromal matrix assembly PMID: 12381269 the posterior stroma of the cornea, which is most vulnerable to lumican-deficiency, demonstrates an early developmental defect in fibril structure and architecture in the Lum-/- genotype PMID: 16786597 a novel role for lumican in the presentation of bacterial lipopolysaccharide to CD14 and host response to this bacterial endotoxin. PMID: 17616530 Lumican is identified as a novel regulator of angiogenesis. PMID: 17632767 Homologous sequence in lumican and fibromodulin leucine-rich repeat 5-7 competes for collagen binding. PMID: 19008226

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Mouse Lumican Protein (C-6His)

Recombinant Mouse Lumican Protein (C-6His)

Product Overview

Target Details

FAQs