Recombinant Mouse KIRREL3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3090
Recombinant Mouse KIRREL3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3090
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Mouse |
Accession | Q8BR86 |
Synonym | 1500010O20Rik, 2900036G11Rik, mKIAA1867, NEPH2, SST4 |
Background | Kin of IRRE-like protein 3 (KIRREL3) also known as nin of irregular chiasm-like protein 3 or nephrin-like protein 2 (NEPH2) is a member of the nephrin-like protein family of transmembrane proteins, which includes NEPH1 (KIRREL) and NEPH3 (KIRREL2). KIRREL3/NEPH2 is expressed in fetalv and adult brain, and also in podocytes of kidney glomeruli. The cytoplasmic domains of KIRREL3/NEPH2 interact with the C-terminus of podocin, also expressed in the podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. Mutations in KIRREL3/NEPH2 are associated with mental retardation autosomal dominant type 4. KIRREL3/NEPH2 expression is turned on in migrating nucleogenesis of the pontine nucleus (PN) neurons only after they enter the presumptive nuclear region. KIRREL3/NEPH2 knockdown disrupted the nuclear organization of PN presumably by changing the migratory behavior of PN neurons inside the nuclear region. Moreover, overexpression of the cytoplasmic region of KIRREL3, which can sequester intracellular signaling of endogenous KIRREL3, resulted in similar phenotypes. Overall, these results suggest KIRREL3 is involved in the nucleogenesis of the PN through the control of neuronal migration inside the nucleus. |
Description | A DNA sequence encoding the mouse KIRREL3 (Q8BR86-1) extracellular domain (Met 1-Ala 535) was expressed, with a C-terminal His tag. |
Source | HEK293 |
Predicted N Terminal | Leu 22 |
AA Sequence | Met 1-Ala 535 |
Molecular Weight | The secreted recombinant mouse KIRREL3 consists of 525 a.a. (pro form) and has a calculated molecular mass of 57.5 kDa. The apparent molecular mass of the recombinant mouse KIRREL3 is approximately 65 kDa in SDS-PAGE under reducing conditions. |
Purity | >96% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells. When cells are added to mouse KIRREL3 coated plates (15 ug/mL, 100 uL/well), > 40% will adhere specifically after 90 minutes at 37 °C. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |