Recombinant Mouse Interleukin-11 Protein (IL11), Active
Beta LifeScience
SKU/CAT #: BLC-05360P
Recombinant Mouse Interleukin-11 Protein (IL11), Active
Beta LifeScience
SKU/CAT #: BLC-05360P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
| Description | Recombinant Mouse Interleukin-11 Protein (IL11), Active is produced by our E.coli expression system. This is a full length protein. |
| Purity | Greater than 97% as determined by SDS-PAGE and HPLC. |
| Endotoxin | Less than 1.0 EU/μg as determined by LAL method. |
| Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine T11 cells is less than 2 ng/ml, corresponding to a specific activity of >5.0x10 5 IU/mg. |
| Uniprotkb | P47873 |
| Target Symbol | IL11 |
| Synonyms | Il11Interleukin-11; IL-11 |
| Species | Mus musculus (Mouse) |
| Expression System | E.coli |
| Tag | Tag-Free |
| Complete Sequence | M+PGPPAGSPR VSSDPRADLD SAVLLTRSLL ADTRQLAAQM RDKFPADGDHSLDSLPTLAM SAGTLGSLQL PGVLTRLRVD LMSYLRHVQW LRRAGGPSLKTLEPELGALQ ARLERLLRRL QLLMSRLALP QAAPDQPVIP LGPPASAWGSIRAAHAILGG LHLTLDWAVR GLLLLKTRL |
| Expression Range | 22-199aa |
| Protein Length | Full Length of Mature Protein |
| Mol. Weight | 19.1 kDa |
| Research Area | Immunology |
| Form | Lyophilized powder |
| Buffer | Lyophilized from a 0.2 µm filtered PBS, pH 7.4 |
| Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
| Target Function | Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation, also in the context of various cancers. Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling'. |
| Subcellular Location | Secreted. |
| Protein Families | IL-6 superfamily |
| Database References | KEGG: mmu:16156 STRING: 10090.ENSMUSP00000092492 UniGene: PMID: 29217821 |
