Recombinant Mouse Interferon alpha 2 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2985
Recombinant Mouse Interferon alpha 2 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2985
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | Fc |
Host Species | Mouse |
Accession | P01573 |
Synonym | Ifa2 |
Background | Type I Interferons (IFNs) are well known cytokines which exert antiviral activity, antitumor activity and immunomodulatory effects. Interferon tau (IFNT), a type I IFN similar to alpha IFNs (IFNA), is the pregnancy recognition signal produced by the ruminant conceptus. |
Description | A DNA sequence encoding the mouse IFNA2 (P01573?) (Cys 24-Glu 190) was fused with the Fc region of human IgG1 at the N-terminus. |
Source | HEK293 |
Predicted N Terminal | Glu |
AA Sequence | Cys 24-Glu 190 |
Molecular Weight | The recombinant mouse IFNA2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 427 a.a. and has a calculated molecular mass of 47.8 kDa. The apparent molecular mass of the monomer is approximately 52 kDa in SDS-PAGE under reducing conditions. |
Purity | >88% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 0.1-0.8 ng/mL.2. Measured by its binding ability in a functional ELISA. Immobilized mouse IFNAR1-His at 10 ug/ml (100 ul/well) can bind mouse Fc-IFNA2, The EC50 of mouse Fc-IFNA2 is 0.8-1.9 ug/ml. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Produced by macrophages, IFN-alpha have antiviral activities. |
Subcellular Location | Secreted. |
Protein Families | Alpha/beta interferon family |
Database References | STRING: 10090.ENSMUSP00000100778 UniGene: Mm.14091 |