Recombinant Mouse Integrin Alpha-X (ITGAX) Protein (MBP&His)

Name: Recombinant Mouse Integrin Alpha-X (ITGAX) Protein (MBP&His)
Brand: Beta LifeScience
SKU: BLC-02423P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: Cluster of differentiation (cd) proteins, Featured cd protein molecules, High-quality recombinant proteins

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Product Overview

Description	Recombinant Mouse Integrin Alpha-X (ITGAX) Protein (MBP&His) is produced by our Baculovirus expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q9QXH4
Target Symbol	ITGAX
Synonyms	Itgax; Integrin alpha-X; CD11 antigen-like family member C; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; CD antigen CD11c
Species	Mus musculus (Mouse)
Expression System	Baculovirus
Tag	N-MBP&C-6His
Target Protein Sequence	FNLDAEKLTHFHMDGAEFGHSVLQYDSSWVVVGAPKEIKATNQIGGLYKCGYHTGNCEPISLQVPPEAVNISLGLSLAAATNPSWLLACGPTVHHTCRENIYLTGLCFLLSSSFKQSQNFPTAQQECPKQDQDIVFLIDGSGSISSTDFEKMLDFVKAVMSQLQRPSTRFSLMQFSDYFRVHFTFNNFISTSSPLSLLGSVRQLRGYTYTASAIKHVITELFTTQSGARQDATKVLIVITDGRKQGDNLSYDSVIPMAEAASIIRYAIGVGKAFYNEHSKQELKAIASMPSHEYVFSVENFDALKDIENQLKEKIFAIEGTETPSSSTFELEMSQEGFSAVFTPDGPVLGAVGSFSWSGGAFLYPSNMRPTFINMSQENEDMRDAYLGYSTALAFWKGVHSLILGAPRHQHTGKVVIFTQESRHWRPKSEVRGTQIGSYFGASLCSVDMDRDGSTDLVLIGVPHYYEHTRGGQVSVCPMPGVGSRWHCGTTLHGEQGHPWGRFGAALTVLGDVNGDSLADVAIGAPGEEENRGAVYIFHGASRQDIAPSPSQRISASQIPSRIQYFGQSLSGGQDLTRDGLVDLAVGSKGRVLLLRTRPILRVSPTVHFTPAEISRSVFECQEQVAPEQTLSDATVCLHIHESPKTQLGDLRSTVTFDLALDHGRLSTRAIFKETKTRALTRVKTLGLNKHCESVKLLLPACVEDSVTPITLRLNFSLVGVPISSLQNLQPMLAVDDQTYFTASLPFEKNCGADHICQDDLSVVFGFPDLKTLVVGSDLELNVDVTVSNDGEDSYGTTVTLFYPVGLSFRRVAEGQVFLRKKEDQQWQRRGQHSLHLMCDSTPDRSQGLWSTSCSSRHVIFRGGSQMTFLVTFDVSPKAELGDRLLLRARVGSENNVPGTPKTTFQLELPVKYAVYTMISSHDQFTKYLNFSTSEKEKTSVVEHRFQVNNLGQRDVPVSINFWVPIELKGEAVWTVMVSHPQNPLTQCYRNRLKPTQFDLLTHMQKSPVLDCSIADCLHLRCDIPSLGILDELYFILKGNLSFGWISQTLQKKVLLLSEAEITFNTSVYSQLPGQEAFLRAQTKTVLEMYKVHNPVP
Expression Range	20-1116aa
Protein Length	Partial
Mol. Weight	165.4 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	Integrin alpha chain family
Database References	KEGG: mmu:16411 STRING: 10090.ENSMUSP00000033053 UniGene: PMID: 29891680 alphaXbeta2 uses the alphaX alphaI domain to bind iC3b on its C3c moiety at one of two sites. PMID: 28292891 Accumulation of lung CD11c+ DCs is significantly higher in both inflammatory and fibrotic phases of the disease but that percentages got reduced in the absence of TGF-beta. PMID: 28557137 The findings of this study indicate that radio-resistant cells, expressing CD11c-GFP+ and located in the CNS, irrespective of their origin, which might be the yolk sac or the periphery, show a phenotype capable of antigen presentation under neuroinflammatory conditions following induction of EAE. PMID: 28421248 These data demonstrated that the CD11c+ lamina propria mononuclear phagocytes from this postinfectious irritable bowel syndrome mouse model were not only able to transfer enteric inflammation to the normal mice but also caused abnormal intestinal function, characterized by epithelial barrier disruption and visceral hyperalgesia. PMID: 28440501 the CD11c promoter was unexpectedly active and triggered Mgat2 deletion within multiple hematopoietic lineages, showed remarkably poor penetrance within native DC populations, and produced almost undetectable levels of green fluorescent protein signal. These findings show that the CD11c promoter is not DC-specific, and extreme care should be taken in the interpretation of data using any mouse created using the CD11c-CRE PMID: 27146521 Studies indicate that the autoimmune phenotype in DEF6 and SWAP-70 knock-out (DKO) mice is characterized by the accumulation of B cell subset that expresses high levels of CD11c and T-bet (ABCs). PMID: 28780965 MyD88 signaling in lysozyme M and CD11c-expressing myeloid cells, as well as in pulmonary epithelial cells, is critical to restore inflammatory cytokine and antimicrobial peptide production, leading to efficient neutrophil recruitment and enhanced bacterial clearance. PMID: 26627460 Results implicate a monocytic origin of CD11c(+) cells in inflamed islets and suggest that therapeutic regulatory T cells directly or indirectly regulate their influx by altering the chemotactic milieu in the islets. PMID: 28550204 Murine CD11c+ T cells analyzed by flow cytometry display markers associated with non-conventional T cell subsets, including gammadelta T cells and invariant natural killer T (iNKT) cells. PMID: 27119555 Data concluded that the ablation of CD11c(hi) dendritic cells provided a subsidiary impact on blood-brain barrier (BBB) integrity and the expression of tight junction/adhesion molecules, thereby leading to exacerbated Japanese encephalitis (JE) progression. PMID: 27638116 CD11c(+) monocyte/macrophages promote chronic Helicobacter hepaticus-induced intestinal inflammation through the production of IL-23. PMID: 26242598 data demonstrate that CD11c(hi) DCs provide a critical and unexpected role to preserve the immune-privileged CNS in lethal neuroinflammation via regulating the differentiation, function, and trafficking of CD11b(+)Ly-6C(hi) monocytes. PMID: 26626303 The generation of mouse line with Flip deficiency in cells that express cre under the CD11c promoter is reported. PMID: 25963626 Probucol effectively retarded atherosclerosis at least partly through lipid-lowering and inhibiting immune maturation of CD11c dendritic cells in STZ-induced diabetic LDLR mice. PMID: 25714599 Dual function of PPARgamma in CD11c+ cells ensures immune tolerance in the airways. PMID: 26062999 CD11c-expressing B cells are located at the T cell/B cell border in spleen and are potent antigen-presenting cells. PMID: 26034175 Our findings suggest that increased numbers of CD11c(+) macrophages and elevated levels of TNF-alpha and MMP-3 in AT and ST may explain the relationship between hyperlipidaemia and OA. PMID: 25693634 Selective ablation of ICOSL in CD11c+ cells, but not in B cells, dramatically ameliorates kidney and lung inflammation in lupus-prone transgenic mice. PMID: 25786178 the role of the aryl hydrocarbon receptor in the function of CD11c cells during respiratory viral infection. PMID: 24519489 provide new insight into how CD11c(+) cells regulate the lymph node vascular-stromal compartment, add to the evolving understanding of functional stromal subsets PMID: 24659690 Data indicate taht tumor necrosis factor receptor superfamily member 14 (TNFRSF14)participated in ovariectomy (OVX)-induced adipose tissue (AT) inflammation via upregulation of CD11c, resulting in metabolic perturbation. PMID: 24287621 The findings identify CD11c macrophages in the lung are likely an important source of IL-23 during ALI, which may be helpful for better understanding of this disease. PMID: 23481504 CD11c+ dendritic cells are essential for de novo induction of Foxp3 expression in naive CD4+ T cells and for airway tolerance. PMID: 23733880 Approximately 26.6% of the cells in DC+ fraction co-expressed CD11c+ and F4/80 markers. PMID: 23146677 Data conclude that both GM-CSF and FLT3L act through PU.1 to activate the 5.3 Kb CD11c proximal promoter in DCs and to induce differentiation of monocyte-derived DCs. PMID: 23284905 down-regulated upon dendritic cell activation through Toll-like receptor triggering PMID: 22445076 Data indicate the amounts of Ptpn6 (Shp1) and MyD88 protein were reduced in dendritic cells of Ptpn6fl/flMyd88fl/flItgax-cre mice. PMID: 23521885 IRF7 regulates TLR2-mediated activation of splenic CD11c(hi) dendritic cells PMID: 22815909 AP-1 composed with JunD and Fra2 protein plays a primary role in enhancing the transcription level of the CD11c gene in dendritic cells PMID: 22990073 CD11c+ cells produce cytokines and/or chemokines required for VCAM-1 upregulation on lung endothelium, which in turn is crucial for Cd11c+ induced mast cell progenitor recruitment and an increase in mast cell numbers. PMID: 22972929 This study showed that, while CD11c(high) DCs initiate helminth-specific adaptive immunity, innate type 2 cells are able to mount an autonomous response to the challenge of parasite infection. PMID: 22851746 airway epithelial NF-kappaB activation is a critical modulator of the adaptive immune response to inhaled antigens via soluble mediator secretion to affect the capacity of CD11c(+) cells to undergo maturation and promote antigen-specific allergic responses PMID: 20581095 Residential macrophages require integrin alphaXbeta2 to control Candida albicans invasion of the brain in vivo. PMID: 22844116 A model where CD23(+) B cells act as antigen transporting cells, delivering antigen to CD11c(+) cells for presentation to T cells is consistent with available experimental data. PMID: 21765910 Our studies provide for the first time evidence that CD11c expression may abrogate the ability to reduce primary virus replication in the eye and TG via higher activities of type 1 interferon and CD8(+) T cell responses. PMID: 21775452 Small intestine CD11c+ CD8+ T cells suppress CD4+ T cell-induced immune colitis. PMID: 21436315 Data show that telmisartan reduced the mRNA expression of CD11c and TNF-alpha, M1 macrophage markers, and significantly increased the expressions of M2 markers, such as CD163, CD209. PMID: 21427223 Depletion of kidney CD11c+ F4/80+ cells impairs the recovery process in ischaemia/reperfusion-induced acute kidney injury. PMID: 20388633 Pulmonary CD11c+ cells are critical for NO2-promoted allergic sensitization via antigenspecifici CD4+ T-cell polarization. PMID: 20659336 findings point to role for CR4 on dendritic cells in triggering B cells during humoral immunity PMID: 20465572 CD11c-positive dendritic cells make a critical downstream contribution to anti-death receptor (DR)5 antitumor activity. PMID: 20505139 CD11c-positive M1-like ATMs can exhibit phenotypic plasticity and that the polarization of these cells between inflammatory and non-inflammatory states is well correlated to the presence of absence of insulin resistance PMID: 20308074 Dendritic cells expressing CD11c and class II major histocompatibility antigens promote high endothelial cell quiescence in vitro and, in vivo, mediate quiescence in expanded lymph nodes. PMID: 20231692 These data demonstrate that CD11c(+) cells play a critical role in protecting the organs from systemic injury caused by a pulmonary endotoxin challenge. PMID: 19948830 Diet-induced obesity increased CD11c in both adipose tissue and blood. PMID: 19910635 CD11c is a useful marker for detection and isolation of pulmonary antiviral cytotoxic T cells following RSV infection. PMID: 16011799 4-1BB stimulation enhanced both primary and memory anti-herpesvirus-1 CD8+ T cell responses, which was mediated by a massive expansion of antigen-specific CD11c+CD8+ T cells PMID: 16524567 CD11c+ dendritic cells-derived osteoclasts are capable of inducing bone loss after adoptive transfer in vivo PMID: 16920972 Results indicate that expression of p150/95 is critical on both T cells as well as other leukocytes for the development of demyelinating disease and may represent a novel therapeutic target for multiple sclerosis. PMID: 17525267

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.