Recombinant Mouse IL-7 Protein (C-6His)

Name: Recombinant Mouse IL-7 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-1675NP
Availability: InStock

BL-1675NP: Greater than 95% as determined by SEC-HPLC. (Regularly tested)

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Product Overview

Description	Recombinant Mouse Interleukin-7 is produced by our Mammalian expression system and the target gene encoding Glu26-Ile154 is expressed with a 6His tag at the C-terminus.
Accession	P10168
Synonym	IL-7; IL-7 interleukin-7; interleukin-7; Lymphopoietin -1; PBGF
Gene Background	Mouse interleukin-7(IL-7) is the member of hemopoietin family which is important to the differentiation, proliferation, and survival of lymphocyte. Mouse IL-7 shares approximately 88% aa sequence identity with rat IL-7 and 58-60% with human, equine, bovine, ovine, porcine, feline and canine IL-7. It is widely expressed in primary and secondary lymphoid tissues cell and stromal epithelial cells of the thymus, bone marrow, and intestines. IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development. It is important for proliferation during certain stages of B-cell maturation. IL-7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2. It is required for optimal T cell-dendritic cell interaction.
Molecular Mass	15.9 KDa
Apmol Mass	22-28 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Biological Activity	Biologically active. Please contact us to obtain bioactivity data.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
Subcellular Location	Secreted.
Protein Families	IL-7/IL-9 family
Database References	KEGG: mmu:16196 STRING: 10090.ENSMUSP00000126219 UniGene: PMID: 29505028 A key role of the IL7 and Interferon type I receptor axis in the regulation of intratumoral t-cell functions and in the development of primary breast tumor growth and metastasis. PMID: 29070614 BMP4 and IL7 appear to be involved in the interaction between intestinal epithelial cells and intestinal epithelial cells and in the mechanism underlying intestinal mucosal barrier dysfunction. PMID: 29436597 This finding warrants future development of IL-21 and IL-7 co-expressing whole-cell cancer vaccines and their relevant combinatorial regimens. PMID: 27571893 both Flt3 ligand (FL) and IL-7 regulate B-cell commitment in a permissive manner: FL by inducing proliferation of Ly6D(+)CD135(+)CD127(+)CD19(-) progenitors and IL-7 by providing survival signals to these progenitors PMID: 27911806 the in vivo biological role of m(6)A modification in T-cell-mediated pathogenesis and reveals a novel mechanism of T cell homeostasis and Il-7 signal-dependent induction of mRNA degradation PMID: 28792938 IL-7/IL-7R signaling pathway plays a possible role in recurrent pregnancy loss by upregulating Th17 immunity while downregulating Treg immunity. PMID: 27767237 this study shows that IL-7 homeostasis is achieved through consumption by multiple subsets of innate and adaptive immune cells PMID: 28723549 lymphatic vessel expansion occurs in two distinct phases; the first wave of expansion is dependent on IL-7; the second phase, responsible for leukocyte exit from the glands, is regulated by lymphotoxin (LT)betaR signaling PMID: 27474071 DNM2 mutations cooperate with Lmo2 T-cell oncogenes by enhancing IL-7 signalling. PMID: 27118408 IL-7 responsiveness in RTE is designed to maximize survival at the expense of reduced proliferation, consistent with RTE serving as a subpopulation of T cells rich in diversity but not in frequency. PMID: 27129922 Expression of IL-7 is beneficial for induction of potent and long-lasting humoral immune responses. PMID: 28100620 findings support a redundant role for adaptive Th17 cell- and innate gammadeltaT17 cell-derived IL17 in bacteria-induced colon carcinogenesis, stressing the importance of therapeutically targeting the cytokine itself rather than its cellular sources PMID: 26880802 functional Gimap5 is required for optimal signaling through TCR and IL-7R in T cells. PMID: 27023180 continuous IL7 signaling was not required for tumor regression, although LIP of naive CD8+ T cells is usually regulated by IL7 PMID: 26880265 IL7 represses the follicular helper T cell gene program. PMID: 26743592 This study uncovers the metabolic mechanisms by which IL-7 tailors the metabolism of memory T cells to promote their longevity and fast response to rechallenge. PMID: 25957683 IL-7 reduced IRAK-M expression and attenuated immune tolerance induced by either LPS or CpGA PMID: 26218271 Poly I:C induces IL-7 production, early inflammatory responses, and characteristic pathologies of SS-like dacryoadenitis in non-autoimmune-prone C57BL/6 mice. PMID: 26658504 Data show that interleukin 7 (IL-7) signaling is a prerequisite for optimal CD4(+) T cell activation. PMID: 26319414 reduced surface expression of IL-7R and concomitant limited responsiveness to IL-7 signals as a common mechanism resulting in reduced cell survival of common lymphoid progenitors and thymocytes at the double-negative to double-positive transition PMID: 26475928 Hair follicle expression of IL-7 was required for CD8+ and CD4+ skin-resident memory T (TRM) cells to exert tropism for the epidermis. In a cutaneous T cell lymphoma model, CD4+ TRM lymphoma cell localization depended on hair follicle-derived IL-7. PMID: 26479922 Data show the contribution of IL-23/IL-23 receptor and IL-7/IL-7 receptor signaling in Th17 and Th1 cell dynamics during experimental autoimmune encephalomyelitis (EAE). PMID: 26223651 IL-7 plays a major role in innate immunity against Citrobacter rodentium infection. PMID: 26034215 The effects of 6-formylindolo (3,2-b) carbazole (Ficz), a ligand of aryl hydrocarbon receptor, on IL-7 expression, colitis and lymphocyte phenotypes are reported. PMID: 25799939 IL-7 critically acts cooperatively with signaling via the pre-TCR and Notch1 to coordinate proliferation, differentiation and TCRalpha recombination during beta-selection. PMID: 25729925 The enhanced thymic reconstitution in the rIL-7/HGFbeta-treated allogeneic BMT recipients results in increased number and functional activities of peripheral T cells. PMID: 24349415 these results suggest that PU.1 and Spi-B activate Btk to oppose IL-7 responsiveness in developing B cells. PMID: 25505273 Results provided evidence that IL-7/IL-7R induce VEGF-D upregulation and promote lymphangiogenesis via c-Fos/c-Jun pathway in lung cancer. PMID: 24115038 OX40 and IL-7 play synergistic, but distinct roles in the homeostatic proliferation of CD4(+) effector memory T cells PMID: 25103720 IL-7 holds promise as a critical cytokine for selectively inducing Tfh cell generation. PMID: 24899182 NF-kappaB has a role in controlling IL-7 responsiveness of quiescent naive T cells PMID: 24799710 IL-12 induces the expression of IL-7 in microglia and macrophages via both IL-12Rbeta2 and IL-12Rbeta1. PMID: 24224652 Ikaros is a central regulator of IL-7 signaling and pre-B cell development PMID: 24297995 our data point toward an unexpected new role for IL-7 as a potential autocrine mediator of lymphatic drainage PMID: 23963040 KGF could up-regulate IL-7 expression through the STAT1/IRF-1, IRF-2 signaling pathway, which is a new insight in potential effects of KGF on the intestinal mucosal immune system. PMID: 23554911 -7 enhances the Th1 response to promote the development of Sjogren's syndrome-like autoimmune exocrinopathy in mice. PMID: 23666710 IRFs activated by lymphocyte adhesion induce IL-7 transcription through ISRE in stromal cells. PMID: 23376291 our results suggest that thymic epithelial cell-derived IL-7 plays a major role in proliferation, survival, and maturation of thymocytes and is indispensable for gammadelta T cell development PMID: 23686483 We have analyzed the discrete contributions of the antibody constant (Fc) and IL-7-binding (Fab) domains to the mechanism. PMID: 23610371 expression of IL-7/IL-7R is strongly correlated with rheumatoid arthritis activity and ligation of IL-7 to IL-7R contributes to monocyte homing, differentiation of osteoclasts, and vascularization in the collagen-induced arthritis effector phase. PMID: 23606539 IL-7 could be an important mediator in arthritic conditions PMID: 22676399 Cessation of the IL-7 response of pre-B cell signaling components is controlled via a cell-autonomous mechanism that operates at a discrete developmental transition marked by transient expression of c-Myc. PMID: 23420891 Interleukin-7, but not thymic stromal lymphopoietin, plays a key role in the T cell response to influenza A virus. PMID: 23189186 Interleukin-7 supports survival of T-cell receptor-beta-expressing CD4(-) CD8(-) double-negative thymocytes. PMID: 23215679 poly I:C boosts the T cell immune response in the lung by inducing local IL-7 production, which in turn, enhances T cell-derived IFN-gamma to promote macrophage recruitment, CXCR3 ligand expression, and T cell infiltration. PMID: 23271706 Data show that lymphatic endothelial cells (LECs) are a prominent source of IL-7 both in human and murine lymph nodes. PMID: 22955921 This is the first demonstration that high levels of IL-7 antagonize Notch-1 signaling and suggest that IL-7 may affect T- versus B-lineage choice in the thymus. PMID: 22899673 Although IL-7 is crucial for naive CD4+ T cell homeostatic proliferation in response to lymphopenia, it has minimal impact on the homeostatic proliferation of regulatory CD4+ (Treg) cells. PMID: 22933631 These observations establish a key role for IL-7 in the complex mechanisms by which immune mediators modulate metabolic functions. PMID: 22768283

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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