Recombinant Mouse IgG1 Protein (102 Cys/Ser)
Beta LifeScience
SKU/CAT #: BLPSN-2599
Recombinant Mouse IgG1 Protein (102 Cys/Ser)
Beta LifeScience
SKU/CAT #: BLPSN-2599
Catalog No.: BLPSN-2599
Product Overview
| Tag | 102 Cys/Ser |
| Host Species | Mouse |
| Accession | P01868 |
| Synonym | IgG1, Igh-4, VH7183 |
| Background | As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. |
| Description | The Fc region of mouse IgG1 (P01868-1) (Val 98-Lys 324) (one aa mutation, 102 Cys / Ser) was expressed and purified. |
| Source | HEK293 |
| Predicted N Terminal | Val 98 |
| AA Sequence | Val 98-Lys 324 |
| Molecular Weight | The recombinant mouse IgG1 Fc consists of 227 a.a. and has a predicted molecular mass of 25.8 kDa. As a result of glycosylation, the apparent molecular mass of rmFc is approximately 32 kDa in SDS-PAGE under reducing conditions. |
| Purity | >97% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 100mM NaAc, 10mM NaCl, 200mM Tris, pH 7.5. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
