Name: Recombinant Mouse HER3 / ERBB3 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2416
Availability: InStock

Recombinant Mouse HER3 / ERBB3 Protein (His Tag)

Catalog No.: BLPSN-2416

Product Overview

Tag	His
Host Species	Mouse
Accession	Q61526
Synonym	ERBB3, ErbB-3, HER3, LCCS2, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, erb-b2 receptor tyrosine kinase 3, FERLK
Background	Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene.ErbB3 is a member of the epidermal growth factor receptor (EGFR/ERBB) family of receptor tyrosine kinases. The kinase-impaired ErbB3 is known to form active heterodimers with other members of the ErbB family, most notably the ligand binding-impaired ErbB2.ERBB3 (Erb-B2 Receptor Tyrosine Kinase 3) is a Protein Coding gene. Diseases associated with ERBB3 include Lethal Congenital Contracture Syndrome 2 and Erythroleukemia, Familial. Among its related pathways are PI3K/AKT activation and RET signaling. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and transferase activity, transferring phosphorus-containing groups. An important paralog of this gene is ERBB4.
Description	A DNA sequence encoding the mouse ERBB3 (Q61526) extracellular domain (Met 1-His 641) was expressed, with a C-terminal His tag.
Source	HEK293
Predicted N Terminal	Ser 20
AA Sequence	Met 1-His 641
Molecular Weight	The secreted recombinant mouse ERBB3 comprises 633 amino acids and has a calculated molecular mass of 70 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 95 kDa in SDS-PAGE under reducing conditions.
Purity	>93% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its binding ability in a functional ELISA.1. Immobilized mouse ErbB3-His at 10 ug/ml (100 ul/well) can bind biotinylated human NRG1 (isoform Beta1) with a linear range of 0.018-0.125 ug/ml.2. Immobilized mouse ErbB3-His at 10 ug/ml (100 ul/well) can bind biotinylated human NRG1 (aa 2-246)-Fc with a linear range of 6.25-100 ng/ml.
Formulation	Recombinant Mouse HER3 / ERBB3 Protein (His Tag) was lyophilized from sterile PBS, pH 7.4
Stability	Recombinant Mouse HER3 / ERBB3 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.