Recombinant Mouse GFR alpha-1 / GDNFR Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-2235

Recombinant Mouse GFR alpha-1 / GDNFR Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-2235
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Product Overview

Tag His
Host Species Mouse
Accession AAB86600.1
Synonym AU042498
Background Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-beta (TGF-â) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.
Description A DNA sequence encoding the extracellular domain (Met 1-Ser 425) of mouse GFRalpha1 isoform beta (AAB86600.1) was expressed with a C-terminal His tag.
Source HEK293
Predicted N Terminal Asp 25
AA Sequence Met 1-Ser 425
Molecular Weight The secreted recombinant mouse GFRalpha1consists of 412 a.a. and has a calculated molecular mass of 46.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.
Purity >98% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.3-1.5 ug/mL in the presence of 40 ng/mL Recombinant Human GDNF.2. Measured by its ability to bind with human GDNF in a functional ELISA.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.
Subcellular Location Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus, trans-Golgi network. Endosome. Endosome, multivesicular body.
Protein Families GDNFR family
Database References
Tissue Specificity Expressed in the brain, in hippocampal neurons (at protein level). Isoform 1 and isoform 2 are expressed in heart, brain, lung, liver, kidney and testis.

Gene Functions References

  1. We further find that overexpression of NANOS2 results in the repression of GFRA1 and PLZF in gonocytes, leading to a delay in GST. On the other hand, loss of NANOS2 results in the up-regulation of GFRA1 and PLZF, indicating a precocious entry of GST. PMID: 29339164
  2. Compromised Survival of Cerebellar Molecular Layer Interneurons Lacking GDNF Receptors GFRalpha1 or RET Impairs Normal Cerebellar Motor Learning. PMID: 28591570
  3. GFRA1 regulates AMPK-dependent autophagy by promoting SRC phosphorylation independent of proto-oncogene RET kinase PMID: 27754745
  4. the neural cell adhesion molecule NCAM is co-expressed and directly interacts with GFRalpha1 in embryonic Purkinje cells. PMID: 28076782
  5. LncRNA033862 is an antisense transcript of the GDNF receptor alpha1 (Gfra1) that lacks protein coding potential and regulates Gfra1 expression levels by interacting with Gfra1 chromatin. PMID: 26962690
  6. These findings collectively demonstrate that GFRalpha1 released by nerves enhances perineural invasion through GDNF-RET signaling and that GFRalpha1 expression by cancer cells enhances but is not required for it. PMID: 24778213
  7. Gfra1 expression in the urinary tract strictly depends on cell-type and specific stage in kidney development. PMID: 23542432
  8. our data demonstrate that RET/GFRalpha signals are dispensable for thymic T cell development in vivo PMID: 23300832
  9. GFRalpha1 is critical for the development and function of the main olfactory system, contributing to the development and allocation of all major classes of neurons and glial cells. PMID: 23197722
  10. Donor-derived GFRalpha1-positive cells formed several aggregates of A(al(aligned)) /morula-like structures in a single spermatogenic cell patch before and on day 14 post-transplant, indicating involvement in the formation of a stable spermatogenic colony. PMID: 22745058
  11. GFRA1 expression is regulated by GDNF and in cultures of isolated seminiferous tubules, we found that GDNF expression and secretion by Sertoli cells is stage-dependent, being maximal in stages II-VI and decreasing thereafter. PMID: 22143971
  12. Histone methylation and acetylation regulate Pou5f1 and Gfra1 in male germ cell. PMID: 20856864
  13. Following ischemic injury, GFR alpha-1 is differentially up-regulated in GFR alpha-1 (+/+) and GFR alpha-1 (+/-) mice. PMID: 12697293
  14. localization pattern of c-kit, Notch-1 and GFRalpha-1 in postnatal, immature and adult testes PMID: 15237212
  15. GFR alpha-1, the receptor for GDNF is strongly expressed by a subset of type A spermatogonia. PMID: 15708562
  16. The role of GFRalpha1 in the developing brain is to promote the differentiation and migration of cortical GABAergic neurons. PMID: 15748846
  17. Data suggest that the combinatorial interactions of the spliced isoforms of GFRalpha, RET and NCAM may contribute to the pleiotropic biological responses. PMID: 15979200
  18. Spermatogonial stem cells expresses GFRA1, but also the stem cell pool is heterogeneous with respect to the level of GFRA1 expression PMID: 16014811
  19. Conditional ablation of GFRalpha1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung's disease phenotype. PMID: 17507417
  20. Gfra1 is expressed dominantly in mouse spermatogonial stem cells; Gfra1 knockdown leads to their differentiation via the inactivation of RET tyrosine kinase, suggesting an essential role for Gfra1 in spermatogonial stem cell regulation. PMID: 17625109
  21. The absence of Gfr alpha1 had no effect on either the appearance of brain-derived fatty acid binding protein (B-FABP) cells or the relative area of the ganglia occupied by B-FABP-immunoreactive cells. PMID: 18551627
  22. The findings point to increased sensitivity of the DAergic system with age and neurotoxic exposure as a result of a genetic reduction of GFR alpha1. PMID: 18822276
  23. The present study clearly establishes that genetic reductions in GFRalpha-1 can contribute to the degenerative changes observed in this system during the aging process. PMID: 18973577
  24. From the data of this study suggested that GFR1 signaling guides the development of a subset of parvalbumin-expressing GABAergic interneurons populating discrete regions of the cerebral cortex. PMID: 19710321


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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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