Recombinant Mouse GFR alpha-1 / GDNFR Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2235
Recombinant Mouse GFR alpha-1 / GDNFR Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2235
Collections: Other recombinant proteins, Recombinant proteins
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Product Overview
Tag | His |
Host Species | Mouse |
Accession | AAB86600.1 |
Synonym | AU042498 |
Background | Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-beta (TGF-â) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells. |
Description | A DNA sequence encoding the extracellular domain (Met 1-Ser 425) of mouse GFRalpha1 isoform beta (AAB86600.1) was expressed with a C-terminal His tag. |
Source | HEK293 |
Predicted N Terminal | Asp 25 |
AA Sequence | Met 1-Ser 425 |
Molecular Weight | The secreted recombinant mouse GFRalpha1consists of 412 a.a. and has a calculated molecular mass of 46.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions. |
Purity | >98% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.3-1.5 ug/mL in the presence of 40 ng/mL Recombinant Human GDNF.2. Measured by its ability to bind with human GDNF in a functional ELISA. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. |
Subcellular Location | Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus, trans-Golgi network. Endosome. Endosome, multivesicular body. |
Protein Families | GDNFR family |
Database References | |
Tissue Specificity | Expressed in the brain, in hippocampal neurons (at protein level). Isoform 1 and isoform 2 are expressed in heart, brain, lung, liver, kidney and testis. |
Gene Functions References
- We further find that overexpression of NANOS2 results in the repression of GFRA1 and PLZF in gonocytes, leading to a delay in GST. On the other hand, loss of NANOS2 results in the up-regulation of GFRA1 and PLZF, indicating a precocious entry of GST. PMID: 29339164
- Compromised Survival of Cerebellar Molecular Layer Interneurons Lacking GDNF Receptors GFRalpha1 or RET Impairs Normal Cerebellar Motor Learning. PMID: 28591570
- GFRA1 regulates AMPK-dependent autophagy by promoting SRC phosphorylation independent of proto-oncogene RET kinase PMID: 27754745
- the neural cell adhesion molecule NCAM is co-expressed and directly interacts with GFRalpha1 in embryonic Purkinje cells. PMID: 28076782
- LncRNA033862 is an antisense transcript of the GDNF receptor alpha1 (Gfra1) that lacks protein coding potential and regulates Gfra1 expression levels by interacting with Gfra1 chromatin. PMID: 26962690
- These findings collectively demonstrate that GFRalpha1 released by nerves enhances perineural invasion through GDNF-RET signaling and that GFRalpha1 expression by cancer cells enhances but is not required for it. PMID: 24778213
- Gfra1 expression in the urinary tract strictly depends on cell-type and specific stage in kidney development. PMID: 23542432
- our data demonstrate that RET/GFRalpha signals are dispensable for thymic T cell development in vivo PMID: 23300832
- GFRalpha1 is critical for the development and function of the main olfactory system, contributing to the development and allocation of all major classes of neurons and glial cells. PMID: 23197722
- Donor-derived GFRalpha1-positive cells formed several aggregates of A(al(aligned)) /morula-like structures in a single spermatogenic cell patch before and on day 14 post-transplant, indicating involvement in the formation of a stable spermatogenic colony. PMID: 22745058
- GFRA1 expression is regulated by GDNF and in cultures of isolated seminiferous tubules, we found that GDNF expression and secretion by Sertoli cells is stage-dependent, being maximal in stages II-VI and decreasing thereafter. PMID: 22143971
- Histone methylation and acetylation regulate Pou5f1 and Gfra1 in male germ cell. PMID: 20856864
- Following ischemic injury, GFR alpha-1 is differentially up-regulated in GFR alpha-1 (+/+) and GFR alpha-1 (+/-) mice. PMID: 12697293
- localization pattern of c-kit, Notch-1 and GFRalpha-1 in postnatal, immature and adult testes PMID: 15237212
- GFR alpha-1, the receptor for GDNF is strongly expressed by a subset of type A spermatogonia. PMID: 15708562
- The role of GFRalpha1 in the developing brain is to promote the differentiation and migration of cortical GABAergic neurons. PMID: 15748846
- Data suggest that the combinatorial interactions of the spliced isoforms of GFRalpha, RET and NCAM may contribute to the pleiotropic biological responses. PMID: 15979200
- Spermatogonial stem cells expresses GFRA1, but also the stem cell pool is heterogeneous with respect to the level of GFRA1 expression PMID: 16014811
- Conditional ablation of GFRalpha1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung's disease phenotype. PMID: 17507417
- Gfra1 is expressed dominantly in mouse spermatogonial stem cells; Gfra1 knockdown leads to their differentiation via the inactivation of RET tyrosine kinase, suggesting an essential role for Gfra1 in spermatogonial stem cell regulation. PMID: 17625109
- The absence of Gfr alpha1 had no effect on either the appearance of brain-derived fatty acid binding protein (B-FABP) cells or the relative area of the ganglia occupied by B-FABP-immunoreactive cells. PMID: 18551627
- The findings point to increased sensitivity of the DAergic system with age and neurotoxic exposure as a result of a genetic reduction of GFR alpha1. PMID: 18822276
- The present study clearly establishes that genetic reductions in GFRalpha-1 can contribute to the degenerative changes observed in this system during the aging process. PMID: 18973577
- From the data of this study suggested that GFR1 signaling guides the development of a subset of parvalbumin-expressing GABAergic interneurons populating discrete regions of the cerebral cortex. PMID: 19710321